We narrowed to 1,247 results for: Ncl

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EGFP-OSBP-PH

Plasmid

#49571

Purpose

expresses the plekstrin homology domain of OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

OSBP-PH (AA 91-179) (OSBP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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p6351 MSCV-CMV-CMV-Flag-HA-NSD3

Plasmid

#31357

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

NSD3 (NSD3 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1

Plasmid

#73408

Purpose

Expresses human AKT1 with a N-terminus HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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SF-U7snRNA-CypA-E4

Plasmid

#190695

Purpose

Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA antisense CypA Exon 4

Use

Lentiviral

Expression

Mammalian

Promoter

U7 promoter

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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p6350 MSCV-CMV-CMV-Flag-HA-ATAD5

Plasmid

#31356

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

ATAD5 (ATAD5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_C (with mCherry)

Plasmid

#72627

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-shCTRL

Plasmid

#181875

Purpose

Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

control shRNA with no known targets in the mouse genome

Use

AAV, Adenoviral, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MTORKinaseDomain

Plasmid

#120708

Purpose

Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MTOR Kinase Domain (MTOR Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 1967-2549

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MTOR HEAT

Plasmid

#120709

Purpose

Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MTOR HEAT 1-9 (MTOR Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 1-409

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-mRuby3

Plasmid

#214923

Purpose

expression vector control - contitutive expression of mRuby33 alone

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mRuby3

Use

Lentiviral

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP1341

Plasmid

#119272

Purpose

control sgRNA in cells lacking rfp

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD/Triple Mutant

Plasmid

#120711

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353, K307A, K310A, R312L

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_N300

Plasmid

#228814

Purpose

Expression of human EHMT1 N-terminal 300 aa only

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1-300 only

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_Ank+SET

Plasmid

#228815

Purpose

Expression of human EHMT1 with deletion of N-terminal 300 aa

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of N-terminal 300 aa

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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