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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc Rheb Omp25

Plasmid

#112764

Purpose

express myc-Rheb-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

myc-Rheb-Omp25 (RHEB Human)

Tags

Omp25 and myc

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1964

Plasmid

#82574

Purpose

High copy number plasmid containing Ple305 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple305

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 23, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Frt-V5-hspB10

Plasmid

#63111

Purpose

Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2A1

Plasmid

#67076

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1 (GTF2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry TFRC_B

Plasmid

#78544

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Expression

Yeast

Promoter

VTC4 (native promoter)

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(369-598)

Plasmid

#135438

Purpose

Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh1

Plasmid

#41927

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

A435S

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_UG3

Plasmid

#133874

Purpose

Expresses UG3 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Tags

helical linker - mEGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (CTD)

Plasmid

#112768

Purpose

express GFP-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (C terminal domain only) (RRAGC Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (CTD)

Plasmid

#112769

Purpose

express Flag-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (C terminal domain only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (GD)

Plasmid

#112770

Purpose

express Flag-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domian only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (GD)

Plasmid

#112767

Purpose

express GFP-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (G domain only) (RRAGC Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (GD)

Plasmid

#112772

Purpose

express Flag-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (CTD)

Plasmid

#112771

Purpose

express Flag-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (C terminal domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagB (CTD)

Plasmid

#112766

Purpose

express GFP-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (C terminal domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 GFP RagB (GD)

Plasmid

#112765

Purpose

express GFP-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (G domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Kdr

Plasmid

#48178

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p5M-PR-B-1-688

Plasmid

#89115

Purpose

mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor-B N-term+DNA binding domain (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker, contai…

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 BMPR2

Plasmid

#67127

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR2 (BMPR2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh3

Plasmid

#41930

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh2

Plasmid

#41928

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf164

Plasmid

#12852

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf164

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 1-373 pBacPAK8

Plasmid

#15328

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 1-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-621 pBacPAK8

Plasmid

#15329

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-621

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-373 pBacPAK8

Plasmid

#15330

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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