We narrowed to 3,399 results for: guide rna expression plasmid

1. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDECKO-mCherry MALAT1_Exon.1

   Plasmid

   #78540

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDECKO-mCherry TFRC_B

   Plasmid

   #78544

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDECKO-mCherry MALAT1_Exon.2

   Plasmid

   #78541

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDECKO-mCherry MALAT1_Exon.4

   Plasmid

   #78543

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDECKO-mCherry MALAT1_Enhancer.4

   Plasmid

   #78539

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDECKO-mCherry MALAT1_Enhancer.3

   Plasmid

   #78538

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDECKO-mCherry MALAT1_Exon.3

   Plasmid

   #78542

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDECKO-mCherry MALAT1_Enhancer.2

   Plasmid

   #78537

   Purpose

   Experimental plasmid

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and U6 (for expressing sgRNA)

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAN057

    Plasmid

    #220053

    Purpose

    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.3846G>A mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKL2315

    Plasmid

    #187876

    Purpose

    Expresses Cre recombinase.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-EAMP

    Plasmid

    #138265

    Purpose

    mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    iCas9 target-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-HTLV

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-TO-g0 (FLP-IN)

    Plasmid

    #236120

    Purpose

    Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    g0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter with two TetR binding sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP942-pX330-Sncg

    Plasmid

    #181774

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Sncg guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AiP926-pX330-Batf3

    Plasmid

    #181772

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Batf3 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP928-pX330-Gpr139

    Plasmid

    #181767

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Gpr139 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP938-pX330-Slc17a7

    Plasmid

    #181775

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Slc17a7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge