We narrowed to 6,370 results for: wus;

1. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

   Plasmid

   #160739

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nGFP-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDA056

   Plasmid

   #131387

   Purpose

   ORFeus expression, WT

   Depositor

   Kathleen Burns

   Article

   Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

   Insert

   ORFeus-Hs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 NLRP1

   Plasmid

   #166823

   Purpose

   Gateway pDONR vector containing NLRP1 (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR3-FLAG-Ubc13-WT

   Plasmid

   #43938

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   ubiquitin-conjugating enzyme E2N (UBE2N Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXB1.Tat(48-57)-Cre recombinase

   Plasmid

   #165042

   Purpose

   Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Mxe Intein-Chitin binding domain and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInducer20 CARD8-HA PAM

   Plasmid

   #169982

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

   Plasmid

   #107939

   Purpose

   pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   mCherry(dpiRNA)::ANI-1(AHPH)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiV_Neo_SIK3_CR

   Plasmid

   #108104

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation)

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_NEUROG3_P2A_Hygro_Barcode

   Plasmid

   #120468

   Purpose

   Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROG3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_NRL_P2A_Hygro_Barcode

    Plasmid

    #120469

    Purpose

    Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NRL (NRL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-NPC1(P691S)-FLAG

    Plasmid

    #164973

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P691S

    Promoter

    EF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Trak2

    Plasmid

    #127622

    Purpose

    N-terminal GFP-tagged mouse Trak2

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak2 (Trak2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. dTAG NLRP1

    Plasmid

    #166825

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_KLF17_P2A_Hygro_Barcode

    Plasmid

    #120503

    Purpose

    Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF17 (KLF17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV_18xTEAD_luc2

    Plasmid

    #173003

    Purpose

    firefly luciferase reporter vector with 18x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    18xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV7.1_3xFLAG-WWC2

    Plasmid

    #172995

    Purpose

    constitutive expression of FLAG-tagged WWC2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC2 (WWC2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDA095

    Plasmid

    #131391

    Purpose

    generation of Tet-On RPE

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti_sgRNA_MS2_neo

    Plasmid

    #118650

    Purpose

    To clone sgRNA for activation dCas9

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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