We narrowed to 60,538 results for: Gene

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pAHYB-GFP

Plasmid

#85074

Purpose

Expression of GFP in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al Yeast. 2014 Apr;31(4):115-25.

Insert

GFP

Expression

Yeast

Promoter

AOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunB

Plasmid

#40349

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

JunB (Jun Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagBFP-PuroR

Plasmid

#80969

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagBFP

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-NanoLuc-PuroR

Plasmid

#80962

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

NanoLuc

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS

Plasmid

#31830

Depositor

Stephen Ekker

Article

Hyatt et al Methods Cell Biol. 1999;59:117-26.

Type

Empty backbone

Promoter

Lac

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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lacI-PT5-gusA-pBAV1k

Plasmid

#30501

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Tags

His

Expression

Bacterial

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pAL1

Plasmid

#197285

Purpose

Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

Plasmid

#187273

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

Anillin A740D, E758K

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTox-EGFPsite1_NGG-PAM (BPK740)

Plasmid

#181746

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)

Use

Selection plasmid

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCR2.1-PBGD

Plasmid

#36869

Depositor

Mario Chin

Insert

PBGD fragment (HMBS Human)

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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221 pCS 3MT DEST

Plasmid

#13070

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

6x Myc Tag

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60b

Plasmid

#21035

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60b (SMARCD2 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-TRAF4

Plasmid

#16375

Depositor

Wafik El-Deiry

Article

Sax et al J Biol Chem. 2003 Sep 19. 278(38):36435-

Insert

TRAF4 (TRAF4 Human)

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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MSGV Hu Acceptor PGK-NGFR

Plasmid

#64270

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P13

Plasmid

#120024

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBSDT-AII

Plasmid

#27179

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

MC1 promoter diphtheria toxin-A fragment

Use

Mouse Targeting

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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PKC beta I CAT

Plasmid

#16380

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-671

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pUG6SP-tTA

Plasmid

#41029

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tTA (tetR-VP16)

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR-A-HYG

Plasmid

#29635

Depositor

David_L Andrews, Scott Gold

Article

Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

Type

Empty backbone

Use

Gateway Cloning

Available Since

Oct. 5, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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CS2 HA-Smad6

Plasmid

#14962

Depositor

Joan Massague

Article

Hata et al Genes Dev. 1998 Jan 15. 12(2):186-97.

Insert

Smad6 (SMAD6 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

HA

Mutation

S18L

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTC-28S15-0X

Plasmid

#20316

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

Psmyc-tetR#28

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pSH1/S-Fvls-p30Casp9-E

Plasmid

#15272

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36)-p30Caspase9 (CASP9 Human)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Synthetic, Mustard Weed)

Tags

FLAG

Promoter

CMV

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pKEN GFP mut2

Plasmid

#20409

Depositor

Stanley Falkow, Raphael Valdivia

Article

Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

Insert

GFP

Expression

Bacterial

Mutation

S65A V68L S72A

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pUC19-Q8DTE3

Plasmid

#103129

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-A7HP89

Plasmid

#103082

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLVTH

Plasmid

#12262

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJFRC5-5XUAS-IVS-mCD8::GFP

Plasmid

#26218

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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