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pLV-EF1a-KIF5A-HA-IRES-Puro

Plasmid

#166952

Purpose

Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Lentiviral

Tags

HA

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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PFD1130w-bio-His

Plasmid

#50823

Purpose

Expresses enzymatically monobiotinylated full length PFD1130w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFD1130w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 R1337P

Plasmid

#21939

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, R1337P

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#226475

Purpose

Improved microglia-targeted transgene expression

Depositor

Hirokazu Hirai

Article

Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
target sequences for miR-9x4 & miR-129-2-3px4 *2
EGFP

Use

AAV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Tags

none

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
SERA4-bio-His

Plasmid

#50816

Purpose

Expresses enzymatically monobiotinylated full length SERA4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Brd9(mouse)-3HA

Plasmid

#248780

Purpose

Express mouse Brd9 in mammalian cells

Depositor

Wenyan Ren

Article

Wu et al Commun Biol. 2025 Dec 1. doi: 10.1038/s4

Insert

brd9 (Brd9 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_hsPum2_hsAdar2_T375G_E488Q

Plasmid

#250538

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2 (E488Q, T375G)

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

hsPum2_hsAdar2_T375G_E488Q (ADARB1, PUM1 Human)

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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p16-mCherry-CYRI-A

Plasmid

#247375

Purpose

Expresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with internal mCherry after proline 16, preserving the proposed N-terminal myristoylation site.

Depositor

Laura Machesky

Article

Le et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Cyri-A (Cyria Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pNP2085

Plasmid

#222731

Purpose

Rep101 Ts - IS621 WT Donor (22bp) with Bba_R0040-KanR Cargo and Rep101 Temp Sensitive

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Donor

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-hCA4-miR122

Plasmid

#244908

Purpose

AAV transgene plasmid encoding human CA4 with miR-122 target site

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

human CA-IV

Use

AAV

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-N-RAM-CytoTape-V5

Plasmid

#239425

Purpose

CytoTape signal monomer for recording Npas4 protein activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

N-RAM promoter

Available Since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-HSE-CytoTape-V5

Plasmid

#239426

Purpose

CytoTape signal monomer for recording HSE promoter transcriptional activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5 (HSPA1A Synthetic)

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

HSPA1A promoter

Available Since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPR V2 SLC31A1_sg2

Plasmid

#244870

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 SLC31A1_sg1

Plasmid

#244869

Purpose

Knockout of human SLC31A1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

SLC31A1 sgRNA (SLC31A1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_JUNO_GFPnes

Plasmid

#240499

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

JUNO (Izumo1r Mouse)

Tags

GFPnes and V5

Expression

Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_IZUMO1-V5_H2B-RFP

Plasmid

#240496

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

IZUMO1 (Izumo1 Mouse)

Tags

H2B-RFP and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_JUNO_H2B-RFP

Plasmid

#240498

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

JUNO (Izumo1r Mouse)

Tags

H2B-RFP and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Ec holA(Y316A)-FLAG-pET(11a)(ACH-)

Plasmid

#238346

Purpose

over-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coli

Depositor

Michael O'Donnell

Insert

C-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)

Tags

3X FLAG

Expression

Bacterial

Mutation

Y316A

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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