167,513 results

1. SuperNova Green/ pRSETB

   Plasmid

   #124839

   Purpose

   bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

   Insert

   SuperNova Green
   

   Tags

   No tag

   Expression

   Bacterial

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-FOXL2-cterm

   Plasmid

   #192893

   Purpose

   Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2 C-term sgRNA (FOXL2 Synthetic)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-jGCaMP8f-WPRE (AAV9)

   Viral Prep

   #176756-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-EF1a-jGCaMP8f-WPRE (#176756). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8f-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   EF1a

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

4. Str-KDEL_CD63-SBP-mCherry

   Plasmid

   #222326

   Purpose

   Synchronize the trafficking of CD63 from the ER.

   Depositor

   Franck Perez

   Article

   Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

   Insert

   Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CNR2-DuET

   Plasmid

   #213214

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CNR2 (CNR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-Neo/Myc PD-L1

   Plasmid

   #121477

   Purpose

   expression of human Myc-PD-L1 in mamalian cell

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TurboID-MCS MSCV-IRES-GFP

   Plasmid

   #207958

   Purpose

   Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

   Depositor

   Timothy Ley

   Article

   Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

   Insert

   TurboID
   

   Use

   Retroviral; Turboid biotin proximity ligase

   Tags

   TurboID

   Expression

   Mammalian

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HIP1R-GFP-FKBP

   Plasmid

   #100752

   Purpose

   Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   HIP1R (HIP1R Human)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

   Plasmid

   #140534

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G) mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Promoter

    hSyn

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    6His, TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SuperNova-N1

    Plasmid

    #131853

    Purpose

    Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kif1bβ-pcDNA3

    Plasmid

    #22534

    Depositor

    William Kaelin

    Article

    Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

    Insert

    Kif1bβ (KIF1B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDR-FOXL2-T2A-tdTomato-PuroTK

    Plasmid

    #192892

    Purpose

    HDR donor for inserting FOXL2-T2A-tdTomato reporter

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2-tdTomato (FOXL2 Human)
    

    Use

    CRISPR

    Mutation

    T2A-tdTomato fluorescent reporter

    Promoter

    PGK

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RED

    Plasmid

    #139835

    Purpose

    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Expression

    Mammalian

    Mutation

    dCas9 protein contains two mutations: D10A and H8…

    Promoter

    tet-inducible CMV

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. STR-KDEL_PSAP-SBP-mCherry

    Plasmid

    #180440

    Purpose

    Co-expresses (via IRES) streptavidin-KDEL and Prosaposin-SBP-mCherry

    Depositor

    Shawn Ferguson

    Article

    Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

    Insert

    Prosaposin (PSAP )
    

    Tags

    SBP-mCherry

    Expression

    Mammalian

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF.hHES1.Ubc.GFP

    Plasmid

    #17624

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (HES1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.Lamp1-GFP

    Plasmid

    #180250

    Purpose

    Mammalian expression of LAMP1

    Depositor

    Marvin Bentley

    Article

    Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

    Insert

    LAMP1 (LAMP1 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CIDAR MoClo Parts Kit

    Plasmid Kit

    #1000000059

    Purpose

    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli

    Depositor

    Douglas Densmore

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only