We narrowed to 6,371 results for: KIT;

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pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2+AtAct2t

Plasmid

#170882

Purpose

Phytobrick - AtACT2t

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

AtACT2t

Expression

Plant

Mutation

426C>T to remove BbsI restriction site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+NOSt

Plasmid

#170885

Purpose

Phytobrick - NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

NOSt

Expression

Plant

Mutation

300C>T to remove BbsI restriction site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD+35St

Plasmid

#170886

Purpose

Phytobrick - 35St

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

35St

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Cas9

Plasmid

#63154

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9

Use

CRISPR; Tol2 middle entry vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCk3

Plasmid

#136697

Purpose

Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-EGFP-FAK

Plasmid

#38194

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-Ms-L-Myc

Plasmid

#26023

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc (Mycl Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK1

Plasmid

#38193

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62 D337, 338, 339A

Plasmid

#38280

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed D337A, D338A, D339A

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pUPD+Red-F

Plasmid

#170874

Purpose

Phytobrick - Red-F Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

Red-F

Use

Luciferase and Synthetic Biology

Mutation

p.S286Y Red mutant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-b5 ER

Plasmid

#38274

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Cytochrome b5 (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Cyb5a Tranmembrane region from 95 to 134 amino ac…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Tags

SV40 NLS

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Single Cassette Destination Vector (pAN2414)

Plasmid

#194220

Purpose

Backbone for constructing single casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro HA-WIPI-1

Plasmid

#38273

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK2

Plasmid

#38201

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUPD2+pCmYLCV911

Plasmid

#170878

Purpose

Phytobrick - pCmYLCV911

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pCmYLCV911

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
1BR9-mCherry

Plasmid

#193864

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherrySTOP

Plasmid

#193866

Purpose

E. coli expression vector for N-ter GFP11 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PGK Promoter (MTK 2) (pAN2822)

Plasmid

#194224

Purpose

Mammalian Toolkit part 2 encoding the PGK promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK Promoter

Use

Synthetic Biology

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a Promoter

Use

Synthetic Biology

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB1_v1.3

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB3_v1.2

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

WPRE terminator

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
1BR9-BFP

Plasmid

#202054

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

BFP

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
1BR9-BFPSTOP

Plasmid

#202055

Purpose

E. coli expression vector for N-ter GFP11 R9 tagged BFP

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

BFP

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
cytoDCIP

Plasmid

#193861

Purpose

Encodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry-GFP1-10

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
1BR9-Lifeact

Plasmid

#193863

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged lifeact peptide.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

Lifeact

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IP HA-Parkin

Plasmid

#38248

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Parkin (PRKN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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