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pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX FynT-SH2

Plasmid

#46430

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynB/FynT (FYN Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1-M7A,H102A

Plasmid

#159168

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A,H102A

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mito-yHS1-M7A,H102A

Plasmid

#159165

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A,H102A

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Opto-GPR37L1

Plasmid

#106020

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR37L1 (GPR37L1 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP12-Actin-C-18

Plasmid

#56244

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Actin (ACTB Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Actin-C-18

Plasmid

#56211

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001101.3 (ACTB Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHR-mCherry-p2A-LIC1 G domain

Plasmid

#74602

Purpose

expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

G168D,Y169D

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NMRK2 gRNA (BRDN0001145387)

Plasmid

#77130

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK2 (NMRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform-2)

Plasmid

#245901

Purpose

Mammalian expression of CDKL5 isoform2 (1-1030) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-1 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CDKL5(kinase)

Plasmid

#245902

Purpose

Mammalian expression of CDKL5 kinase domain (1-311) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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