We narrowed to 10,236 results for: ada

1. EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

   Plasmid

   #191144

   Purpose

   Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1:EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-HA-hTRAF3

   Plasmid

   #44032

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   S393G

   Available Since

   April 4, 2013

   Availability

   Academic Institutions and Nonprofits only

3. EB3-3xmNeonGreen-N1

   Plasmid

   #129605

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGADT7-αC

   Plasmid

   #197650

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-2 αC
   

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Ser677T substitution

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-AlkB deltaN11

   Plasmid

   #73622

   Purpose

   Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

   Depositor

   Catherine Drennan

   Article

   Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   AlkB (alkB E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deletion of first 11 AA

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-M2-TLR2

   Plasmid

   #12291

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I and H202Y; signal peptide (aa1-23) replaced …

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CAG-miRFP680-P2A-GFP

   Plasmid

   #197164

   Purpose

   Expresses the protein of miRFP680-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP680-P2A-GFP
   

   Use

   AAV

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-EZH2

   Plasmid

   #222528

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   EZH2 (EZH2 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-LOV-VP16

    Plasmid

    #42499

    Purpose

    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

    Depositor

    Ricardo Dolmetsch

    Article

    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

    Insert

    FKF1 (N-LOV, G128D mutant) fused with VP16
    

    Tags

    F-type VP16 activation domain and HA tag

    Expression

    Mammalian

    Mutation

    G128D 1-168aa

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

    Plasmid

    #191140

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

    Plasmid

    #159583

    Purpose

    Luciferase reporter for ribosome pausing

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    XBP1u pause site
    EIF2S3 5UTR
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC25

    Plasmid

    #177319

    Purpose

    Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

    Depositor

    Rafal Tomecki

    Article

    Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

    Insert

    TtCutA 240-603
    

    Tags

    6xHis-SUMOTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-YTHDC2

    Plasmid

    #222558

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    YTHDC2 (YTHDC2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-cT3G-cERP2-NELFE

    Plasmid

    #222559

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    NELFE (NELFE Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

    Plasmid

    #191146

    Purpose

    Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdnf.t1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Expression

    Mammalian

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pPB EF1a CCR6-eLOV-TEVcs-Gal4

    Plasmid

    #173119

    Purpose

    Expresses CCR6 eLOV receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-Gal4
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3xmNeonGreen_UtrCH

    Plasmid

    #129607

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Utrophin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

    Plasmid

    #191170

    Purpose

    EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (plant)
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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