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Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-tetoff-mVenus-PICdc42
Plasmid#91876PurposePI(WT): Photo inhibitable (blue light sensitive)DepositorInsertVav2 (VAV2 Human)
UseTagsmVenusExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smurf1 C699A
Plasmid#11753DepositorInsertSMURF1 (SMURF1 Human)
UseTagsFlagExpressionMammalianMutationC699A: catalytically inactivePromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC12A9
Plasmid#131897PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A9 (SLC12A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
NC16 pCDNA3.1 FLAG NRF2
Plasmid#36971DepositorInsertNRF2 (NFE2L2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825)
Plasmid#214862PurposeExpression of iCre(R297T) in striatal medium spiny neuronsHas ServiceAAV PHP.eBInsertiCre(R297T)
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F1
Plasmid#202520PurposeExpressing human E2F1 protein with FLAG-tagDepositorInsertE2F1 (E2F1 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HTR1B-Tango
Plasmid#66405PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR1B (HTR1B Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE155
Plasmid#118505Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80R
Plasmid#118350PurposePlasmid expresses hHSF1 with K80 in DBD mutated to R.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD
Plasmid#204359PurposeAAV vector for coexpression of miniDq and KORD under the control of human synapsin promoterDepositorInsertminiDq-mCherry-P2A-HA-KORD
UseAAVTagsHA (for KORD) and mCherry (for miniDq)ExpressionMutationFor miniDq, the third intracellular loop (ICL3) o…PromoterhSynAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100852PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoterDepositorHas ServiceAAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHygR2
Plasmid#200071PurposehygR selection nls-mScarlet marked rapid RMCE integration vectorDepositorTypeEmpty backboneUseRecombination mediated cassette exchangeTagsExpressionWormMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC6A1_STOP
Plasmid#161408PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC6A1 (SLC6A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)
Viral Prep#100852-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (#100852). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRGECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sp-HPV-TM(ICAM)
Plasmid#196510PurposeExpress HPV antigen epitope with ICAM transmembrane domainDepositorInserta HPV epitope with CD8a stalk linker and ICAM TM domain
UseLentiviralTags3xflag tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY405
Plasmid#172694PurposePunc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neuronsDepositorInsertrab-3 (rab-3 Nematode)
UseTagsPunc-25 and mcherryExpressionWormMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM-intron 3x UAS attB
Plasmid#206360Purposeincludes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequencesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mSTING
Plasmid#214157PurposeTransient expression of STINGDepositorInsertSTING (Sting1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ATN1
Plasmid#16179DepositorInsertAtrophin 1 (ATN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
R777-E033 Hs.DUSP1
Plasmid#70317PurposeGateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for native or N-terminal fusions)DepositorInsertDUSP1 (DUSP1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-EGFP
Plasmid#209057PurposeGateway-compatible entry vector, with insert of the EGFP geneDepositorInsertEGFP
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_WDR81:1-1941
Plasmid#210993PurposeMammalian expression of full-length human WDR81. C-terminal HaloTag.DepositorInsertWDR81:M1-A1941 (WDR81 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-UTY-F
Plasmid#40617DepositorInsertUTY (UTY Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1Available sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only