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HERV-Kcon-RT for Split-PE (pJUL3120)

Plasmid

#190107

Purpose

Expresses HERV-Kcon RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

HERV-Kcon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ266E

Plasmid

#161521

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9(D10A)-PmCDA1-UGI

Use

CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

Tags

3X FLAG, NLS

Expression

Plant

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

Viral Prep

#104495-PHPeB

Purpose

Ready-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Promoter

CAG

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only
ITPKA-mTurquoise2

Plasmid

#137811

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA (ITPKA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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[GG][Pin]

Plasmid

#97191

Purpose

Pin nuclease domain with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pin domain

Use

Synthetic Biology

Tags

Pum cloning site

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

Plasmid

#79902

Purpose

Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

3xFLAG_Twin_Strep_EZH2

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

PGK1

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-RCC1-polyA

Plasmid

#59762

Purpose

Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

RCC1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-dSa-VPR mini.

Plasmid

#99686

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe(SV40)-Cytochrome C-GFP

Plasmid

#41184

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C-GFP

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
shBIRC5 # 2

Plasmid

#42554

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 Y287A

Plasmid

#32824

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y287A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMBEC6

Plasmid

#187598

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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RP2-EGFP

Plasmid

#86073

Purpose

A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLX_305_OVA_Blasticidin

Plasmid

#238124

Purpose

Enables expression of Ovalbumin with Blasticidin selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

Ovalbumin (OVAL Chicken)

Expression

Mammalian

Promoter

Human PGK

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc- GFP-Flag

Plasmid

#82471

Purpose

Expresses myc-GFP-Flag

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-GFP-Flag

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Article

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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