We narrowed to 9,583 results for: SUB
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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225063PurposeExpression of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Xlone Puro SNAP-eGFP-BASU
Plasmid#218452Purposeexpresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integrationDepositorInsertSNAP-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_NLS-Halo-eGFP-BASU
Plasmid#218449Purposeexpressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP
Plasmid#184819PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.DepositorInsertQF2-pA-he1.1:YFP
UseCRISPR; Backbone for cloning of homology arms (fo…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only