167,564 results

1. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

   Plasmid

   #154826

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   mCherry
   NeoR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-3XHA-EGFP-OMP25

   Plasmid

   #83356

   Purpose

   For tagging mitochondria with HA epitopes (HA-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XHA-EGFP-OMP25
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EB3-mScarlet-I

   Plasmid

   #98826

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT_TO_DESTFLAGHA

   Plasmid

   #26361

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Type

   Empty backbone

   Tags

   FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAM1579

   Plasmid

   #40240

   Depositor

   Susan Golden

   Article

   Andersson et al Methods Enzymol. 2000;305:527-42.

   Insert

   Km Resistance, neutral site II integration platform for Synechococcus
   

   Use

   Cyanobacteria cloning vector

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-ddMSK1

   Plasmid

   #165603

   Purpose

   Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, Synthetic, S. Pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-KDEL_SBP-EGFP-LAMP2

   Plasmid

   #222321

   Purpose

   Synchronize the trafficking of LAMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IB-pSu9-HaloTag7-mGFP

   Plasmid

   #184905

   Purpose

   Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   Presequence of Fo-ATPase subunit 9
   

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-Ii_VSVG-SBP-EGFP

   Plasmid

   #65300

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. K785R Smarca4-sfGFP

    Plasmid

    #107057

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-KDEL_CD9-SBP-mCherry

    Plasmid

    #222329

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. minCMV-1xmNG-dPCP

    Plasmid

    #231092

    Purpose

    Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    1xmNG-dMCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-KDEL_CD9-SBP-EGFP

    Plasmid

    #222328

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH41822

    Plasmid

    #48021

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV-CAG-FLEX-SomArchon

    Plasmid

    #126943

    Purpose

    Cre-dependent expression of SomArchon under CAG promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGM65879

    Plasmid

    #153214

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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