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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAGFP

Plasmid

#58255

Purpose

U6 promoter-driven single guide RNA complementary to eGFP

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAGFP cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
c3GIC9n

Plasmid

#62192

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9 (D10A)

Use

CRISPR

Tags

HA

Mutation

D10A substitution (Cas9n)

Promoter

TRE3G

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pY018 (pcDNA3.1-hPdCpf1)

Plasmid

#69990

Purpose

Expresses humanized PdCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPdCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3045(gRNA XI-3)

Plasmid

#73287

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEn-C1.1

Plasmid

#61479

Purpose

Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

Depositor

Holger Puchta

Article

Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_INT

Plasmid

#68436

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGEL030

Plasmid

#137899

Purpose

STU2.0 SpyCas9 Csy4 system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Lmo

Plasmid

#126501

Purpose

L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPH-T2A-GFP-shP53

Plasmid

#102895

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pY012 (pcDNA3.1-hCMtCpf1)

Plasmid

#69984

Purpose

Expresses humanized CMtCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hCMtCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGEL029

Plasmid

#137898

Purpose

STU2.0 SpyCas9 RZ system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTX209

Plasmid

#89269

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01 and OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Promoter

mouse U6

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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