We narrowed to 74,529 results for: Tro;

1. pCIBN-hTRF2-tagRFP-T

   Plasmid

   #103812

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hTRF2: deletion of amino acids 1-42 and 476 compa…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR1A-HA-Jag1

   Plasmid

   #46052

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Jag1 (Jag1 Rat)
   

   Use

   Entry vector

   Tags

   HA tag

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pJET1.2_Gli3_Anti-Sense

   Plasmid

   #124440

   Purpose

   Plasmid for anti-sense in situ probe in vitro transcription

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Insert

   GLI-Kruppel family member GLI3 (Gli3 Mouse)
   

   Use

   In situ probe

   Promoter

   T7

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-plk1-AKAP

   Plasmid

   #132967

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
   

   Expression

   Mammalian

   Mutation

   C terminus of full-length Plk1 fused to the peric…

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-hs-HRAS

   Plasmid

   #52730

   Purpose

   retroviral expression of human HRAS

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   HRAS (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCIBN-tagBFP-hNCL

   Plasmid

   #103805

   Purpose

   BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hNCL (CIB1, NCL Mustard Weed, Human)
   

   Expression

   Mammalian

   Mutation

   hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-EYFP-hNCL

    Plasmid

    #103806

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hNCL (CIB1, NCL Mustard Weed, Human)
    

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p53 S23A targeting vector

    Plasmid

    #12167

    Depositor

    Tyler Jacks

    Article

    MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    Ser23 mutation (S23A) in exon 2 and puromycin gen…

    Available Since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-bio-myc-mCE 2-210

    Plasmid

    #112715

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6/TO-shEsr1-CMV-TetR-eGFP

    Plasmid

    #233298

    Purpose

    DOX-inducible U6 driven shRNA against mouse Esr1

    Depositor

    Dayu Lin

    Article

    Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-bio-myc-mCE 211-597

    Plasmid

    #112716

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-210 (triphosphatase domain)

    Promoter

    CMV

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

    Plasmid

    #202826

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid

    #202825

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKate2-DrCavin1a in pT3TS-Dest

    Plasmid

    #194294

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    In vitro transcription of mrna

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKHH030_sgCtrl#2

    Plasmid

    #174143

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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