We narrowed to 5,998 results for: gca

Showing: 3141 - 3160 of 5998 results

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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ITM2B_Halo_C_allele

Plasmid

#178160

Purpose

Donor vector for endogenous tagging of human ITM2B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ITM2B Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAPsg

Plasmid

#154344

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAP sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R1

Plasmid

#124209

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86202

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLG-Sty

Plasmid

#33149

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

Sty1 site upstream of intron was cut/filled/ligat…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
PX459-CDKN1A

Plasmid

#217457

Purpose

For CDKN1A (p21) knockout

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for CDKN1A

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-HDAC1-shRNA3

Plasmid

#90243

Purpose

Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

Depositor

Roland Friedel

Insert

HDAC1 (HDAC1 Human)

Use

Lentiviral

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 sgCGAS_1

Plasmid

#241454

Purpose

Delete CGAS

Depositor

Richard Possemato

Insert

sgCGAS_1 (CGAS Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI) promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-24xMS2

Plasmid

#238915

Purpose

Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

Depositor

Jian-Hui Jiang

Article

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

iRFP670-24xMS2

Use

Lentiviral

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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VPS35 shRNA2

Plasmid

#163859

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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