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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1-IRES2-EGFP

Plasmid

#170820

Purpose

human GABAA receptors alpha1 subunit and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-D50 lamin A

Plasmid

#17653

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

D50 lamin A (LMNA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50 (50 aa internal deletion in globular tai…

Promoter

CMV

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_H1_Gal4VP64

Plasmid

#169918

Purpose

Expression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch1-GL4VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM1 (PITPNM1 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.1

Plasmid

#139478

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1

Expression

Mammalian

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b His-p97

Plasmid

#169020

Purpose

Expresses His-p97 in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-GFP

Plasmid

#40125

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NQO1-Flag

Plasmid

#61729

Purpose

Expresses human NQO1 Flag tagged at C-terminus

Depositor

Yosef Shaul

Article

Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

Flag

Expression

Mammalian

Promoter

cmv

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pB-mTSPAN4-mCherry

Plasmid

#210330

Purpose

Overexpression of mTSPAN4

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN4 (Tspan4 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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msfGFP-Cx43

Plasmid

#69024

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Article

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR F87E)

Plasmid

#242846

Purpose

Bacterial expression of TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine, F8…

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Tags

CFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR(T/A) (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGGAselect

Plasmid

#195714

Purpose

pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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