We narrowed to 45,591 results for: CaS

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pCaSpeR-act5cB-LexAQF

Plasmid

#61310

Purpose

Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2_AD

Expression

Insect

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pST1374-dCas9-GCN4

Plasmid

#113026

Purpose

Expresses dCas9-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D10A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Six1 (CT#664)

Plasmid

#13876

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Six1 (Six1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
p2301y-tdtomato-dCas9_DegronDead_TPLN188

Plasmid

#205409

Purpose

dCas9 guided repressor

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

dCas9_DegronDead_TPLN188

Expression

Plant

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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RCAS.Sfi myr-Akt1

Plasmid

#196374

Purpose

Akt1 with a myristoylation signal in avian expression vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

myr-Akt1

Use

Retroviral; Avian

Tags

HA and myristoylation

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cNkx2.5 (CT#800)

Plasmid

#13951

Depositor

Cliff Tabin

Article

Smith et al Development. 2000 Sep . 127(17):3671-81.

Insert

Nkx2.5 (NKX2-5 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pBLO43.3.-6_human ProCas9Flavi-6

Plasmid

#121630

Purpose

U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAGT8162 (AATG_dttLbCas12a-i_TTCG)

Plasmid

#192350

Purpose

L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

AATG_dttLbCas12a-i_TTCG

Use

CRISPR

Mutation

D156R; E925Q

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP2(2)

Plasmid

#136061

Purpose

G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12c2-noncoding

Plasmid

#120877

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12c2

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-CCHD (p130Cas)

Plasmid

#145133

Purpose

Expresses CCH domain from p130Cas with an mCherry tag

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

p130Cas CCH domain (BCAR1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti Cas9/SB100

Plasmid

#155294

Purpose

Lentiviral expression of Cas9 and SB100

Depositor

Martin Carroll

Insert

SB100X-P2A-spCas9

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_ACTB-i1 sgRNA / hSpCas9

Plasmid

#172825

Purpose

Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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