We narrowed to 35,837 results for: CaS

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MSCV-shCasp2-IRES-GFP

Plasmid

#52061

Purpose

stable knockdown of Caspase-2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Caspase-2 shRNA (CASP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg1

Plasmid

#124856

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

R661A+K1003H

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9 GW STOP

Plasmid

#183196

Purpose

Destination vector

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV0

Plasmid

#116096

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV0

Expression

Mammalian

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMS10: pTns(AvCAST)_ΔTnsD

Plasmid

#168143

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-NG-nCas9

Plasmid

#163551

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-mFndc3b

Plasmid

#126443

Purpose

Expresses mouse Fndc3b circular RNA

Depositor

Jeremy Wilusz

Article

Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

Insert

Fndc3b (Fndc3b Mouse)

Expression

Mammalian

Mutation

circFndc3b exons are separated by a miniature int…

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1-Pct5.1-crRNA(dadR)

Plasmid

#191634

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-CasE-p300-Flag

Plasmid

#106275

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE-p300

Use

CRISPR

Tags

E tag, NLS and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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PX390 Flavobacterium columnare Cas9

Plasmid

#68327

Purpose

Flavobacterium columnare Cas9

Depositor

Feng Zhang

Insert

FcCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Promoter

U6

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS (A) cTbx3 del2 (CT#645)

Plasmid

#13921

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 del (TBX3 Chicken)

Use

Retroviral; Avian expression

Mutation

Truncated Tbx3. Putative dominant negative.

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only