We narrowed to 7,375 results for: ef1a

1. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97311

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mc2 neo EF1a

   Plasmid

   #206220

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-BC-EF1a-Puro

   Plasmid

   #127170

   Purpose

   PCR template for EF1a-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-EGFP-RSKL

   Plasmid

   #170165

   Purpose

   Mammalian Expression of Ef1a-EGFP-RSKL

   Depositor

   Christopher Moxham

   Insert

   EGFP-RSKL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RSKL

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-Tmx4-V5

   Plasmid

   #120236

   Purpose

   Expresses V5-tagged Tmx4 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Tmx4 (Tmx4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMPUW_6.10_M3_Ef1a-HA-gateway-SV40

   Plasmid

   #128446

   Purpose

   lentiviral destination vector with Ef1a promoter, N-terminal HA tag, and SV40 promoter

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, N-terminal HA tag, and SV40 promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV-tetO-EF1a-GFP

   Plasmid

   #187645

   Purpose

   Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites

   Depositor

   Martin Carroll

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-Itprip-V5

   Plasmid

   #120241

   Purpose

   Expresses V5-tagged Itprip in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Itprip (ITPRIP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-EF1a-NucGFP

    Plasmid

    #184696

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV_EF1a_CDK1-P2A-Hygro_Barcode

    Plasmid

    #170221

    Purpose

    Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-V5-Sec61B

    Plasmid

    #120244

    Purpose

    Expresses V5-tagged Sec61B in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Sec61B (Sec61b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mc2 blast EF1a

    Plasmid

    #206223

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97312

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-EF1a-KLF4

    Plasmid

    #35389

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Klf4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-EF1a-CDreV

    Plasmid

    #140133

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CDreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1a-2xSV40_NLS-CeNLuc

    Plasmid

    #135952

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40

    Plasmid

    #128447

    Purpose

    lentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoter

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, C-terminal HA tag, and SV40 promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-EF1alpha-HA-TFAP4

    Plasmid

    #110158

    Purpose

    Lentiviral vector for expression of human TFAP4 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-DIO-HB

    Plasmid

    #37452

    Depositor

    Edward Callaway

    Insert

    Histone, 2A element, rabies B19 glycoprotein
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

21. MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

    Plasmid

    #170170

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.WT

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Ef1a-QCP-3xBFP

    Plasmid

    #158206

    Purpose

    Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    QCP under the Elfa promoter and three BFP tags
    

    Use

    Synthetic Biology

    Tags

    3xBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Elfa

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EF1a-OCT4

    Plasmid

    #35391

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

    Plasmid

    #194459

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-pEF1a-rtTAc

    Plasmid

    #172121

    Purpose

    Constitutive expression of InteinC-rtTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-rtTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-EF1a-V5-Vrk2

    Plasmid

    #120240

    Purpose

    Expresses V5-tagged Vrk2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Vrk2 (Vrk2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cEF1a-LSL-GFP

    Plasmid

    #135672

    Purpose

    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF1alpha-rTetR-bGHpA

    Plasmid

    #172285

    Purpose

    Constitutive expression of reverse tetracycline-controlled transactivator

    Depositor

    Patrik Verstreken

    Insert

    rTetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EF1alpha

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

    Plasmid

    #170169

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge