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PMT-mKate2_2
Plasmid#203786PurposeBrightness calibrationDepositorInsertPMT-mKate2 dimer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
PMT-mCherry2_2
Plasmid#203784PurposeBrightness calibrationDepositorInsertPMT-mCherry2 dimer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
PMT-mScarlet_2
Plasmid#203780PurposeBrightness calibrationDepositorInsertPMT-mScarlet dimer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
PMT-mCherry_2
Plasmid#203782PurposeBrightness calibrationDepositorInsertPMT-mCherry dimer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVKG1-BSD
Plasmid#108680PurposeBlasticidin resistant cassette for knock-in by VIKING methodDepositorInsertSV40pro-BSD-polyA
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Myotilin-C-14
Plasmid#55316PurposeLocalization: Cytoskeleton, Excitation: 399, Emission: 456DepositorInsertMyotilin (MYOT Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS mouse IRS3 promoter
Plasmid#11444DepositorInsertIRS-3 promoter (Irs3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-10His-mStayGold (E138D)
Plasmid#211363PurposeMammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionMammalianMutationE138DPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
V245 pCEP-4HA B56alpha
Plasmid#14532DepositorInsertPPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-SiT-N-15
Plasmid#55325PurposeLocalization: Golgi TGN, Excitation: 399, Emission: 456DepositorInsertSiT (ST6GAL1 Human)
UseTagsmTagBFP2ExpressionMammalianMutationaa 1-45 of SiTPromoterCMVAvailable sinceOct. 24, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/HisD-rsTagRFP
Plasmid#31927DepositorInsertrsTagRFP
UseTagsHisExpressionBacterialMutationF84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSWEET-bgaB
Plasmid#210272PurposeXylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.DepositorInsertbgaB
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
V247 pCEP 4HA B56gamma1
Plasmid#14534DepositorInsertPPP2R5C B56 gamma1 regulatory subunit of PP2A (PPP2R5C Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVKG1-Puro
Plasmid#108670PurposePuromycin resistant cassette for knock-in by VIKING methodDepositorInsertPURO
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-10His-StayGold
Plasmid#211362PurposeMammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
UseTags10xHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry1-N1
Plasmid#31928DepositorInsertPAmCherry1
UseTagsExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromotercmvAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry1-C1
Plasmid#31929DepositorInsertPAmCherry1
UseTagsExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-PCNA-19-SV40NLS-4
Plasmid#55320PurposeLocalization: Nucleus, Excitation: 399, Emission: 456DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-TFR-20
Plasmid#55327PurposeLocalization: Transferin Receptor, Excitation: 399, Emission: 456DepositorInsertTFR (TFRC Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-EGFP-Rac1-wt
Plasmid#12980DepositorInsertRac1 (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Endosomes-14
Plasmid#55293PurposeLocalization: Endosomes, Excitation: 399, Emission: 456DepositorInsertEndosomes (RHOB Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUdioFRTeYFPW
Plasmid#133998Purposeexpresses eYFP upon coexpression with the recombinase flippase (Flp)DepositorInserteYFP
UseLentiviral; Flp/frtTagsExpressionMammalianMutationinverted and flanked by two incompatible FRT site…PromoterUbCAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 9
Plasmid#36107DepositorInsertG protein subunit Gamma 9
UseTagsYFPExpressionMammalianMutationHIs tag between YFP and insertPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
V249 pCEP-4HA B56delta
Plasmid#14536DepositorInsertPPP2R5D B56 delta regulatory subunit of PP2A (PPP2R5D Human)
UseTags4 HAExpressionMammalianMutationR210HPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
V248 pCEP 4HA B56gamma3
Plasmid#14535DepositorInsertPPP2R5C B56 gamma3 regulatory subunit of PP2A (PPP2R5C Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
V250 pCEP-4HA B56epsilon
Plasmid#14537DepositorInsertPPP2R5C B56 epsilon regulatory subunit of PP2A (PPP2R5E Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKS235
Plasmid#191855PurposeNegative selection marker for MosSCIDepositorInsertpeel-1 toxic protein for negative selection against extrachromosomal array
UseTagsExpressionWormMutationPromoterhsp-16.41Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Vinculin-23
Plasmid#55334PurposeLocalization: Focal Adhesions, Excitation: 399, Emission: 456DepositorInsertVinculin (VCL Human)
UseTagsmTagBFP2ExpressionMammalianMutationV234L in Vinculin.PromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
nonphosphorylatable Mis12-targeted Aurora B FRET sensor
Plasmid#45229DepositorInsertnon phosphorylatable Mis12-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationT-A mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-rat PKA-RIIbeta-mEGFP
Plasmid#45530DepositorInsertPKA regulatory subunit RII beta tagged by mEGFP
UseWith chicken beta actin promoter and wpreTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro rat IRS-3
Plasmid#11450DepositorInsertIRS-3 (Irs3 Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
BiFC3
Plasmid#190048PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
UseTagsExpressionYeastMutationPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC4
Plasmid#190049PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)DepositorInsertYemVenus (ATG-aa173-238)
UseTagsExpressionYeastMutationPromoterMet3Available sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiFC2
Plasmid#190047PurposeBiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)DepositorInsertYemVenus (aa1-172 - TAA)
UseTagsExpressionYeastMutationPromoterMet3Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
BiFC1
Plasmid#190046PurposeBiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)DepositorInsertYemVenus (aa1-172)
UseTagsExpressionYeastMutationPromoterMet3Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Tev-Halo tag
Plasmid#117124PurposeC-terminal Tev Halo-TagDepositorInsertTev-Halo-tag Tag
UseGolden gate donor vector for gene targeting in ba…TagsTev-Halo-TagExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCDNA3.1)
Plasmid#118041PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCDNA3.1)
Plasmid#118038PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCAGGS)
Plasmid#118042PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS*11-yfp0
Plasmid#53241Purposeexpress yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPLtetO1Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Cenp-B-targted Aurora B FRET sensor
Plasmid#45230DepositorInsertnonphos Cenp-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationt-a mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2s-EGFP
Plasmid#118040PurposeGABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminusDepositorInsertGABRG2 (Gabrg2 Rat)
UseTagsEGFP and cMyc epitope EQKLISEEDL inserted between…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro rat IRS-3
Plasmid#11362DepositorInsertIRS-3 (Irs3 Rat)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXTK058 [Con-Pro_sacB-Bacillus_PartType8]
Plasmid#229295PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_sacB-Bacillus_PartType8DepositorInsertsacB Promoter (Bacillus subtilis)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK039 [Con-Pro_sacB-Bacillus_PartType5]
Plasmid#229276PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_sacB-Bacillus_PartType5DepositorInsertsacB Promoter (Bacillus subtilis)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS853
Plasmid#228236PurposeBxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing padDepositorInsertsmCherry
EYFP
UseTagsHA and PuroRExpressionMammalianMutationPromoterTRE3GV and noneAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only
