We narrowed to 4,908 results for: PIR

1. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP21-1A(sfGFP)

   Plasmid

   #126968

   Purpose

   Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECT3

   Plasmid

   #75450

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECT5

   Plasmid

   #75451

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECM1

   Plasmid

   #75445

   Purpose

   Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJUMP41-2A(sfGFP)

   Plasmid

   #126985

   Purpose

   Level 2 vector. sfGFP reporter. Origin R6K (pir conditional).

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECT1

   Plasmid

   #75446

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pECT1B

   Plasmid

   #75447

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pECT1D

   Plasmid

   #75448

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECT1E

    Plasmid

    #75449

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-CD4 [mu5A8] (Recombinant Antibody)

    Antibody

    #212327-rAb

    Purpose

    Anti-CD4 (Human) recombinant mouse monoclonal antibody

    Depositor

    Melina Fan

    Article

    Burkly et al J Immunol. 1992 Sep 1;149(5):1779-87.

    Recommended Applications

    Western Blot

    Reactivity

    Human

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available Since

    June 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

12. Anti-Jagged [D5] (Recombinant Antibody)

    Antibody

    #201864-rAb

    Purpose

    Anti-Jagged (Human) recombinant scFv-Fc-fusion

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Recommended Applications

    Immunocytochemistry

    Reactivity

    Human

    Source Species

    Human

    Isotype

    IgG1

    Trial Size

    Available to purchase

    Available Since

    Nov. 8, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. pAbaMCi-GFP-BsaI_pJMP2748

    Plasmid

    #208888

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Pir dependent origin of replication

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM441

    Plasmid

    #61271

    Purpose

    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23a-LIR-1 D1-D2

    Plasmid

    #12103

    Depositor

    Pamela Bjorkman

    Article

    Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

    Insert

    Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acids 1−198*

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tn7 integration plasmid

    Plasmid

    #154134

    Purpose

    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Inserts

    left and right end of Tn7 arm
    Spectinomycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Spectinomycin resistance gene promoter and no

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EcoFlex MoClo Extension, Volume I

    Plasmid Kit

    #1000000253

    Purpose

    Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

    Depositor

    Mikhail Shapiro

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

20. pLacIq-Wasabi

    Plasmid

    #86112

    Purpose

    mWasabi reporter gene driven by LacIq promoter

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Insert

    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pLacIq

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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