We narrowed to 568 results for: prime editing

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SauCas9 PE2*

Plasmid

#169851

Purpose

Mammalian Expression, sauCas9 based prime editor

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SaCas9-N580A-M-MLV

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2*

Plasmid

#169850

Purpose

Mammalian Expression, SpyCas9 prime editor, optimized NLS

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

spCas9 PE*

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2-3HA

Plasmid

#169853

Purpose

Mammalian Expression, SpyCas9 prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-M-MLV-3HA

Tags

3XHA tag and bpSV40 NLS

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Bxb1

Plasmid

#182142

Purpose

Expression of human codon-optimized Bxb1

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

human codon optimized Bxb1

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-pegRNA-puro backbone

Plasmid

#214085

Purpose

Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Expression

Mammalian

Promoter

EF1-a and hU6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

Plasmid

#177182

Purpose

plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

pegRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12342_PEAR-GFP_2in1

Plasmid

#177184

Purpose

PEAR-GFP plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LMNA_mScarlet-I_Donor

Plasmid

#178092

Purpose

HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA-mScarlet-I HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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