Addgene

We narrowed to 26,133 results for: sta

Showing: 301 - 320 of 26133 results

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pcDNA3-10His-StayGold

Plasmid

#211362

Purpose

Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Use

Tags

10xHis-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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6X-STAT-synthetic luciferase

Plasmid

#37392

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

six STAT consensus sites

Use

Luciferase

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mStayGold

Plasmid

#227251

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-neo

Plasmid

#32426

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

Flag-mCherry-T2A-GFP-T2A-neo

Use

Tags

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4B (Lenti)

Plasmid

#222691

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTAB182_K

Plasmid

#146777

Purpose

Mammalian Expression of HsTAB182

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTAB182 (TNKS1BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBR-Lent-STAT5A-CA

Plasmid

#170662

Purpose

Lentiviral expression of constitutively active STAT5A.

Depositor

Alexander Ruthenburg

Article

Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

Insert

STAT5A (STAT5A Human)

Use

Lentiviral

Tags

Expression

Mutation

H298R and S710F

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Use

Tags

Expression

Insect

Mutation

Promoter

Actin5c

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLEGFP-Y705F-STAT3

Plasmid

#71445

Purpose

Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Y705F

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
CRISPR-StAR4BN (Lenti)

Plasmid

#222692

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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BAP1-wt (siRNA resistant)

Plasmid

#108439

Purpose

Stable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1641C>T, c.1644T>A, c.1647T>C, c.1650C…

Promoter

CMV

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-STAT1

Plasmid

#11987

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Jun 26. ():.

Insert

STAT1 Y701F (STAT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA

Plasmid

#52347

Purpose

Integration cassette to create p1-Short-UL*(TGA)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

leu*

Use

P1 integration vector

Tags

Expression

Bacterial

Mutation

changed S2F, A3V, Q180*

Promoter

UCS from pGKL2 ORF5

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Article

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-mStayGold

Plasmid

#227252

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR1 (Retro)

Plasmid

#222687

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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