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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,452 results for: Mpl;

Showing: 7951 - 8000 of 13452 results

  4. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

    Plasmid
    #157692
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157692-plasmid-map-sequence-id-307834

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  14. pAC8_MF-PH-10His-Cter (VE5631)

    Plasmid
    #161802
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10 His tag
    Tags
    10 His
    Expression
    Insect
    Promoter
    p10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    161802-plasmid-map-sequence-id-324122

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  15. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid
    #139770
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal TwinStrep tag
    Tags
    3C - TwinStrep tag
    Promoter
    PH
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139770-plasmid-map-sequence-id-324179

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  16. pAC8_MF-p10-10His-Cter (VE5742)

    Plasmid
    #139776
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10His tag
    Tags
    10 His
    Expression
    Insect
    Promoter
    PH
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139776-plasmid-map-sequence-id-324157

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  17. pAC8_MF-p10-Flag-Cter(VE5738)

    Plasmid
    #161798
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Flag tag
    Tags
    Flag
    Expression
    Insect
    Promoter
    p10
    Available Since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161798-plasmid-map-sequence-id-324175

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  19. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid
    #139769
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    mCherry fluorescent protein
    Expression
    Insect
    Promoter
    PH or p10
    Available Since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139769-plasmid-map-sequence-id-324116

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  22. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  23. pGAPTrap-mtagtdTom_IRESMuro

    Plasmid
    #82355
    Purpose
    Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    mtagTandemTomato-IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82355-plasmid-map-sequence-id-156569

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  27. pMP108 Nanog-Venus-2a-mCherry

    Plasmid
    #159743
    Purpose
    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.
    Depositor
    Sergi Regot
    Article
    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d
    Insert
    Nanog homeobox (Nanog Mouse)
    Use
    Mouse Targeting
    Tags
    Venus-2a-mCherry
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159743-plasmid-map-sequence-id-330621

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  30. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

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  37. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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Showing: 7951 - 8000 of 13452 results