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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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SlugBE4max

Plasmid

#163799

Purpose

Expresses AncBE4max and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Blat-BSD

Plasmid

#163791

Purpose

The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNICE HA-mSYD1B

Plasmid

#59362

Purpose

Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1B (Syde2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA4n96

Plasmid

#123640

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 4n96

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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FH95pUp95GW (B4)

Plasmid

#74013

Purpose

lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL-Stat5a1*6

Plasmid

#130669

Purpose

Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

Plasmid

#199357

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTW102

Plasmid

#115945

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 DDd-L7Ae

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Nanog-pA-pgk-hph

Plasmid

#74915

Purpose

Mammalian expression of mNanog

Depositor

Jose Silva

Article

Theunissen et al Curr Biol. 2011 Jan 11;21(1):65-71. doi:

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-miSFIT-1x

Plasmid

#124690

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0510

Plasmid

#124001

Purpose

Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Tags

Flag

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

Plasmid

#100979

Purpose

Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

Depositor

Heinrich Leonhardt

Article

Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

Insert

c-Myc NLS-FKBP-Venus N-T2A-mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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[GFP][IRES][Bla]

Plasmid

#97194

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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