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pTa-HH7-2

Plasmid

#124997

Purpose

To make transgenic mice for HH7-2 TCRa

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH7-2 TCRa Tg

Use

Mouse Targeting

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTa-HH5-1

Plasmid

#124995

Purpose

To make transgenic mice for HH5-1 TCRa

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1 TCRa Tg

Use

Mouse Targeting

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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BSP609

Plasmid

#122254

Purpose

expression vector for mTFP under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mTFP

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNfs1

Plasmid

#102967

Purpose

Suppression of mNfs1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNfs1

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pWJPrP34

Plasmid

#22107

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP exon 3 EcoR1 fragment

Use

Construction of targeting vector

Mutation

Added a Cla1 site right before translation start …

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pK-11

Plasmid

#22094

Depositor

Gail Martin

Article

Meyers et al Nat Genet. 1998 Feb . 18(2):136-41.

Insert

Frt-PGKNeo-Frt-LoxP

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pInducer20-Blast

Plasmid

#109334

Purpose

pInducer20 with added blasticidin resistance

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Type

Empty backbone

Use

Lentiviral; Inducible expression

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-EF1α-Dest

Plasmid

#133299

Purpose

The pT3-EF1α destination vector without any insert which can be used for LR reaction.

Depositor

Xin Chen

Article

Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#89573

Purpose

Expresses iCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iCre

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDawn

Plasmid

#43796

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pRL623

Plasmid

#58494

Purpose

helper plasmid for bacterial conjugal DNA transfer

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Insert

encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pMusheLL

Plasmid

#47023

Purpose

Mouse PV L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Article

Handisurya et al Virology. 2012 Nov 25;433(2):385-94. doi

Insert

Mu L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-LOV2

Plasmid

#81009

Purpose

Expression of LOV2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

NTOM20

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag RbBP5

Plasmid

#15550

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

Plasmid

#50022

Purpose

expresses Membrane tagged G-CaMP3 in cells expressing Cre

Depositor

David Anderson

Article

Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

Insert

G-CaMP3

Use

AAV

Tags

MARCKS sequence (MGCCFSKT)

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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