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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEYFP-NR1a

Plasmid

#17928

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)

Tags

EYFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Article

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-CIBN-eGFP-CAAX-Puro

Plasmid

#240804

Purpose

Constitutive expression of CIBN-eGFP

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

CIBN-eGFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-SopF

Plasmid

#135174

Purpose

Expresses an mCherry-SopF fusion in mammalian cells

Depositor

Leigh Knodler

Article

Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

Insert

SopF (STM1239 Salmonella enterica serovar Typhimurium)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTW2082

Plasmid

#236576

Purpose

ISYmu1 PDS3 gRNA12 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pSLQ10704

Plasmid

#183957

Purpose

U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

SKSKSO array

Use

Lentiviral

Tags

EF1a-Puro-T2A-TagBFP

Expression

Mammalian

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Vim-SNAP-tag2

Plasmid

#226518

Purpose

Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Vimentin-SNAP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDOXOFF 99xGly

Plasmid

#211346

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2A

Plasmid

#17924

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

NMDAR2A (Grin2a Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-MS2coatProtein

Plasmid

#103832

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mCherry-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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