178,328 results
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Plasmid#163612PurposeRetro and Lentiviral transduction of primary human germinal center B cellsDepositorInsertGaLV MTR envelope
ExpressionMammalianPromoterCMVAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)
Viral Prep#100854-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-FLEX-DTR-GFP
Plasmid#124364Purposeconditional expression of a diphtheria toxin receptor (DTR)–GFP fusion proteinDepositorHas ServiceAAV2InsertDTR-GFP
UseAAVTagsGFPPromoterChicken B-actinAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Voltron-ST (AAV1)
Viral Prep#119036-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR
Plasmid#116731PurposeLentiviral expression of EGFRDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS-G12D
Plasmid#116423PurposeLentiviral expression of KRAS G12DDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mNeonGreen
Plasmid#98875PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsert3xNLS
TagsmNeonGreenExpressionMammalianPromoterCMVAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-45b(+)-Tn5
Plasmid#112112PurposeExpresses hyperactive Tn5 transposase for next generation sequencing library preparationDepositorInsertTn5
TagsHis6ExpressionBacterialMutationE54K E110K P242A L372PAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
SaBE4-Gam
Plasmid#100809PurposeExpresses SaBE4-Gam in mammalian cellsDepositorInsertSaBE4-Gam
ExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Parts Kit
Plasmid Kit#1000000059PurposeCollection of standardized parts for combinatorial design and expression tuning in E. coli.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden Gate (MoClo)Available SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
shh10
Plasmid#64867PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.DepositorInsertshh10 cap
UseAAVMutationI319V, N451D, D532NAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensDepositorInsertmCherry
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-puro
Plasmid#51240PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-C1
Plasmid#54605PurposeLocalization: C1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneTagsmCerulean3ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NLS
Plasmid#39319DepositorInsertmCherry-3xNLS
Tags3xSV40LT NLSExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-A
Plasmid#38132DepositorAvailable SinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only