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We narrowed to 53,797 results for: ide

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Showing: 3201 - 3220 of 53797 results

  1. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

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  3. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  4. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  5. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72383-plasmid-map-sequence-id-136200

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  6. pET28a-RupA_T1-NHis

    Plasmid
    #109259
    Purpose
    NRPS T domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T1
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 917..1025 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109259-plasmid-map-sequence-id-211504

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  7. pET28a-RupA_R-NHis

    Plasmid
    #109257
    Purpose
    NRPS R domain with N-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_R
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    contains aa 2038..2443 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109257-plasmid-map-sequence-id-211503

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  9. pET29b-RupA_T2-R-CHis

    Plasmid
    #109258
    Purpose
    NRPS di-domain containing TR domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_T2-R
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1939..2443 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109258-plasmid-map-sequence-id-211511

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  10. pET29b-RupA_C2-A2-T2-R-CHis

    Plasmid
    #109256
    Purpose
    NRPS module containing CATR domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_C2-A2-T2-R
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1023..2443 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109256-plasmid-map-sequence-id-214798

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  11. pET29b-RupA_C1-A1-T1-CHis

    Plasmid
    #109255
    Purpose
    NRPS module containing CAT domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_C1-A1-T1
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1..1025 of WP_015523502.1
    Promoter
    T7
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109255-plasmid-map-sequence-id-214294

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  13. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170015-plasmid-map-sequence-id-335287

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  17. pYHY2

    Plasmid
    #216108
    Purpose
    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT0294 signal peptide
    Expression
    Bacterial
    Promoter
    BfP1E6 promoter
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216108-plasmid-map-sequence-id-434369

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  18. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Promoter
    P1TDP-A21
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  20. SpyLigase

    Plasmid
    #51722
    Purpose
    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SpyLigase
    Tags
    His6 Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51722-plasmid-map-sequence-id-251770

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Showing: 3201 - 3220 of 53797 results