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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,497 results for: multi

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Showing: 3201 - 3220 of 18497 results

  1. pNO286-3

    Plasmid
    #107746
    Purpose
    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
    Depositor
    Jeffrey Tabor
    Publication
    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb
    Inserts
    CcaS
    Ho1
    PcyA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Deleted AA 223 and 512
    Promoter
    J23106 and J23108
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107746-plasmid-map-sequence-id-209202

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  3. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140220
    Purpose
    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1
    Use
    CRISPR
    Tags
    miniVPR-mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140220-plasmid-map-sequence-id-291993

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  6. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. pLSLGFP.R

    Plasmid
    #51501
    Purpose
    Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP
    Use
    Plant t-dna plasmid
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    51501-plasmid-map-sequence-id-261239

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  10. pNTI194

    Plasmid
    #84266
    Purpose
    Expresses Avi-tagged RPL1 and humanized birA
    Depositor
    Nicholas Ingolia
    Publication
    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    RPL1 (RPL10A Human, (*see below))
    humanized birA
    Tags
    Avi tag, Flag, Gly/Ser linker, and TEV site
    Expression
    Mammalian
    Mutation
    R33A, A34E (*see below)
    Promoter
    CMV and P2A peptide
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84266-plasmid-map-sequence-id-160813

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  12. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid
    #52381
    Purpose
    BAC recombineering generated construct, used as a reporter for OCT4 expression
    Depositor
    Jacob Hanna
    Publication
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP-2A-puro
    OCT4 (POU5F1 Human)
    Expression
    Mammalian
    Mutation
    deleted -3194 - -1768 upstream from OCT4 ATG
    Promoter
    OCT4
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    52381-plasmid-map-sequence-id-82024

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  14. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Publication
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58540-plasmid-map-sequence-id-90629

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  16. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    167547-plasmid-map-sequence-id-332733

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Showing: 3201 - 3220 of 18497 results