We narrowed to 9,561 results for: nls

1. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC439

   Plasmid

   #195237

   Purpose

   Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ECFP-Nucleus-7

   Plasmid

   #55346

   Purpose

   Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   ECFP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. EBFP2-Nucleus-7

   Plasmid

   #55249

   Purpose

   Localization: Nuclear Localization, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   EBFP2-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. Strain: C321.ΔA

   Bacterial Strain

   #68306

   Purpose

   Genomically recoded strain of E. coli (no genomic TAG)

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Bacterial Resistance

   Bleocin (zeocin)

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. rEc.13.delA

   Bacterial Strain

   #69495

   Purpose

   Recoded strain from MG1655, prfA deleted

   Depositor

   Michael Jewett

   Article

   Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

   Bacterial Resistance

   Ampicillin

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCJ.559

   Bacterial Strain

   #69493

   Purpose

   Strain with recoded TAG and genomic deletions for improved incorporation

   Depositor

   Michael Jewett

   Article

   Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

   Bacterial Resistance

   Ampicillin

   Species

   Eschericia coli

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCSnERT(#186)

    Plasmid

    #184057

    Purpose

    to express cyclofen-inducible fusions in mammalian cells or fish eggs

    Depositor

    Sophie Vriz

    Insert

    nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Target-AID (pRZ762)

    Plasmid

    #131300

    Purpose

    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Nucleus-7

    Plasmid

    #55110

    Purpose

    Localization: Nucleus, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    mCherry-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. DsRed2-Nucleus-4

    Plasmid

    #55839

    Purpose

    Localization: Nucleus, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    DsRed2-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. mPlum-Nucleus-7

    Plasmid

    #55991

    Purpose

    Localization: Nucleus, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    mPlum-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. mRuby-Nucleus-7

    Plasmid

    #55875

    Purpose

    Localization: Nucleus, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    mRuby-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Dronpa-Nucleus-9

    Plasmid

    #57290

    Purpose

    Localization: Nucleus, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    Dronpa-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. HcRed1-Nucleus-7

    Plasmid

    #55843

    Purpose

    Localization: Nucleus, Excitation: 588, Emission: 618

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    HcRed1-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dendra2-Nucleus-7

    Plasmid

    #57734

    Purpose

    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Tags

    Dendra2-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits