We narrowed to 76,692 results for: tra

1. tet-pLKO shGATA6 puro

   Plasmid

   #72615

   Purpose

   Expresses an inducible short hairpin targeting human GATA6 sequence

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   short hairpin GATA binding protein 6
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Tight

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 deltaN47 Beta-catenin

   Plasmid

   #19287

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-47

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. amilCP_Orange chromoprotein

   Plasmid

   #117850

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP_Orange
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   64V,65G, BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 deltaN89 Beta-catenin

   Plasmid

   #19288

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-89

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

5. meffBlue chromoprotein

   Plasmid

   #117845

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, meffBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-BHMT-I/G

   Plasmid

   #104442

   Purpose

   Autophagy cargo assay

   Depositor

   Carol Mercer

   Article

   Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

   Insert

   BHMT (BHMT Human)
   

   Use

   Tags

   GFP-myc GFP is from Aequorea victoria and GST

   Expression

   Mammalian

   Mutation

   N43A in GST (in a potential KFERQ-like sequence).…

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-SEP-Glur2 (MCA10)

   Plasmid

   #64941

   Purpose

   mammalian expression of rat GluR2

   Depositor

   Jeremy Henley

   Article

   Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. proE-cad670-Luc

   Plasmid

   #42083

   Depositor

   Kumiko Ui-Tei

   Article

   Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

   Insert

   E-cadherin (CDH1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   contains E-cadherin promoter region from -670 to …

   Promoter

   E-cadherin

   Available since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHT-6xR.ads-PxylA-DI

   Plasmid

   #47386

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   ads
   dxs
   idi
   

   Use

   Tags

   6xR and His

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac and PxylA

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lyn-FKBP-FKBP-CFP (LF2C)

    Plasmid

    #20149

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-FKBP-FKBP-CFP (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid

    #65262

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-GNPTAB

    Plasmid

    #78108

    Purpose

    Mammalian expression of human GNPTAB with C terminal myc tag

    Depositor

    Thomas Braulke

    Article

    Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

    Insert

    GNPTAB (GNPTAB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. TA1-Tango

    Plasmid

    #66508

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TA1 (TAAR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Use

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)mGAT1-0-GFP

    Plasmid

    #41662

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal hydrophobic isoleucine residue added a…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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