We narrowed to 24,472 results for: CRISPR
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Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH2502
Plasmid#84379PurposeExpression of dcas9 D10A H840A from a TetR-regulated uvtetO promoterDepositorInsertdcas9
UseCRISPRExpressionBacterialMutationAspartate at position 10 changed to Alanine, Hist…Promoteruv15tetOAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-CDDR
Plasmid#149355PurposeCRISPR/Cas9-based dual-fluorescent DSB repair reporterDepositorInsertsCDDR Reporter
Blasticidin resistance
UseLentiviralExpressionMammalianPromoterEF-1a and hPGKAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLP857_α-syn:oScarlet KI donor
Plasmid#239403PurposeAAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-synDepositorInsertoScarlet
UseAAV and CRISPRTagsoScarletExpressionMammalianAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9D10A_GFP
Plasmid#44720PurposeCo-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9D10A-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationD10A mutation- nickase mutantPromoterCAGAvailable SinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianPromoterTRE3GAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT51
Plasmid#223423PurposeT-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6 (for expressing sgRNA)Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-vpr
Plasmid#89985Purposeinducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locusDepositorInsertdCas9-VPR
ExpressionMammalianPromoterTRE-tightAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT48
Plasmid#223420PurposeT-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sg-A
Plasmid#83807PurposeExpress sgRNA targeting SA siteDepositorInsertSgRNA
UseCRISPRAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
sg14x(MS2) MUC4.1
Plasmid#101153PurposegRNA with 14 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
SQT1665
Plasmid#78744PurposeHuman expression plasmid for LbCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only