We narrowed to 67,690 results for: nin

1. F2RL1-DuET

   Plasmid

   #213235

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   F2RL1 (F2RL1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. F2RL2-DuET

   Plasmid

   #213236

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   F2RL2 (F2RL2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CD97-DuET

   Plasmid

   #213206

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CD97 (ADGRE5 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CCRL2-DuET

   Plasmid

   #213205

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCRL2 (CCRL2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C5A-DuET

   Plasmid

   #213192

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   C5A (C5AR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ADRA2C-DuET

   Plasmid

   #213179

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2C (ADRA2C Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-His8-aCat(660-865)

   Plasmid

   #203335

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-865 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-His8-aCat(660-885)

   Plasmid

   #203334

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-885 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRESneo2 mycBioID-Tollip M240A,F241A

   Plasmid

   #178055

   Purpose

   myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   Tags

   mycBioID

   Expression

   Mammalian

   Mutation

   Changed Methionine 240 to Alanine and changed Phe…

   Promoter

   CMV

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. human TLNRD1-4H pET151

    Plasmid

    #159386

    Purpose

    Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
    

    Use

    Tags

    his-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    TLNRD1 4-helix domain (residues 143-273)

    Promoter

    T7

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUS_LC_charge_RtoK

    Plasmid

    #139127

    Purpose

    express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Add K and D to FUS LC so it has the same charge p…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. csd6_13-330_E110A/pET28b(+) N-tag

    Plasmid

    #71354

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Glutamic acid 110 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. csd6_13-330_Y133A/pET28b(+) N-tag

    Plasmid

    #71356

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 133 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

15. csd6_13-330_C176A/pET28b(+) N-tag

    Plasmid

    #71360

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Cysteine 176 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

16. csd6_13-330_H160A/pET28b(+) N-tag

    Plasmid

    #71359

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 160 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

17. csd6_13-330_W158A/pET28b(+) N-tag

    Plasmid

    #71358

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tryptophan 158 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

18. csd6_13-330_H155A/pET28b(+) N-tag

    Plasmid

    #71357

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 155 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. csd6_13-330_Y132A/pET28b(+) N-tag

    Plasmid

    #71355

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 132 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pBullet-cyt-n

    Plasmid

    #53065

    Purpose

    destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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