We narrowed to 25,718 results for: nes

1. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-luxOFP

   Plasmid

   #229950

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxOFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFZ19

   Plasmid

   #36184

   Depositor

   Daniel Voytas

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

   Type

   Empty backbone

   Use

   Plant t-dna vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   XVE

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-luxKate

   Plasmid

   #229951

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-luxKOk

   Plasmid

   #229949

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKOk
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304 Luciferase-V5 blast

   Plasmid

   #98580

   Purpose

   Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMX/cyclinB1 (17)

   Plasmid

   #26060

   Depositor

   Jonathon Pines

   Article

   Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDR FLIP43 GPS1

    Plasmid

    #101084

    Purpose

    GA biosensor expression plasmid

    Depositor

    Wolf Frommer, Alexander Jones

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    GPS1
    

    Use

    Tags

    6HIS and cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pG108 - K

    Plasmid

    #178039

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Insert

    Kanamycin resistance
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ErmAM has been replaced with Kanamycin for select…

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Use

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Julien Berro, Thomas Pollard

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAureus-TnCAM

    Plasmid

    #199807

    Purpose

    vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

    Depositor

    Steve Salipante

    Article

    Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

    Insert

    transposon cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC-1-StUbi-6

    Plasmid

    #197710

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StUbi promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSWEET-bgaB

    Plasmid

    #210272

    Purpose

    Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

    Depositor

    Eric Brown

    Article

    Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

    Insert

    bgaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PxylA

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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