We narrowed to 9,350 results for: pho;

1. pCMV5-Flag-Ago2-FDM1

   Plasmid

   #72208

   Purpose

   Mammalian expression of aa 1-372 of human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Δ 373-860

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCMV5-Flag-Ago2-FDM2

   Plasmid

   #72209

   Purpose

   Mammalian expression of aa 1-517 of human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Δ 518-860

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCMV5-Flag-Ago2-FDM4

   Plasmid

   #72211

   Purpose

   Mammalian expression of aa 373-860 of human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Δ 1-372

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCMV5-Flag-Ago2-FDM5

   Plasmid

   #72212

   Purpose

   Mammalian expression of aa 518-860 of human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Δ 1-517

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Flag-M4-AR-W741C-S650A

   Plasmid

   #171252

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-M4-AR-W741C-S650D

   Plasmid

   #171253

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX RASGAP(C)-SH2

   Plasmid

   #46431

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Gap/RASGAP(C) (RASA1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 339-452

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2-TBG-FLAG-mRIPK1-S321A

    Plasmid

    #115342

    Purpose

    Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321A

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p21-S208C/S242R/M694V-MEFV

    Plasmid

    #134706

    Purpose

    Lentiviral expression of human MEFV S208C/S242R/M694V after doxycycline administration

    Depositor

    Thomas Henry

    Article

    Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mutation

    p.S208C/S242R/M694V

    Promoter

    TRE2 (Dox-inducible)

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX RASGAP(NC)-SH2

    Plasmid

    #46433

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(NC) (RASA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 171-448

    Promoter

    Tac

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MEK1C218

    Plasmid

    #164639

    Purpose

    Bacterial expression plasmid for His6-MEK1C218

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S218C/C277S/C376S

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2-TBG-FLAG-mRIPK1-S321E

    Plasmid

    #115343

    Purpose

    Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid

    #69116

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 682-688 (region containin…

    Promoter

    CMV

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR221-ITGB1(no stop)

    Plasmid

    #215453

    Purpose

    Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Silent mutations added to disrupt shRNA binding a…

    Promoter

    none

    Available since

    Sept. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pENTR2b-ITGB1(YYFF)

    Plasmid

    #215451

    Purpose

    Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Mutations in the NPxY sites Y783F, Y795F; Silent …

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pENTR2b-ITGB1(WT)-mRuby2

    Plasmid

    #215446

    Purpose

    Gateway entry vector with integrin beta1 tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    mRuby2

    Expression

    Mutation

    Silent mutations added to disrupt shRNA binding a…

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid

    #236225

    Purpose

    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells

    Depositor

    Chiaki Murakami

    Article

    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

    Insert

    SAMD8 (SAMD8 Human)
    

    Use

    Tags

    Hexa histidine tag (6xHis-tag) and enterokinase s…

    Expression

    Insect

    Mutation

    sterile alpha motif domain at N-terminal (1-231 b…

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-SET(1-218)

    Plasmid

    #234707

    Purpose

    expression of GFP in fusion with a C-terminally truncated SET protein

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    expresses only the N-terminal SET part (1-218)

    Promoter

    CMV

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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