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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1

Plasmid

#45903

Depositor

Louise Larose

Article

Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

Insert

Nck-1 (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
PKC eta WT

Plasmid

#21244

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pFRT_DESTFLAGHA

Plasmid

#26360

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG metap2

Plasmid

#32004

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

metap2 (Metap2 )

Tags

FLAG

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

Plasmid

#191795

Purpose

vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13d

Use

AAV and CRISPR

Expression

Mammalian

Mutation

A134V, A140V, A141V, A143V

Promoter

EFS, hU6

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMX-PGC1b

Plasmid

#1032

Depositor

Bruce Spiegelman

Article

Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

Insert

PGC-1 beta (Ppargc1b Mouse)

Tags

Gal4

Expression

Mammalian

Available Since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
pEG302 22aa SunTag NtDRMcd nog

Plasmid

#115488

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMyosin-PSmOrange

Plasmid

#31921

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Myosin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK3

Plasmid

#23635

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK3 (DYRK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDest472 SHP-1 WT

Plasmid

#21804

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3_cAMPFIRE-H

Plasmid

#182281

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd5-1D4

Plasmid

#42267

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 5 (Fzd5 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-eGFP-NMHC-IIAΔtailpiece

Plasmid

#35689

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Added stop codon after codon 1928, eliminating th…

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pLL3.7-EF-EYFP-YAP1_5SA-PolyA

Plasmid

#112285

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSUMO3

Plasmid

#52260

Purpose

Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd1

Plasmid

#23227

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd1 (RND1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only