We narrowed to 3,302 results for: grna design
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Plasmid#112244PurposeEncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.DepositorInsertdCas9-NS3-NLS/VPR
TagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mouse genome-wide lentiviral CRISPR gRNA library version 2
Pooled Library#67988PurposeKnockout library targeting 18,424 mouse genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human genome-wide lentiviral CRISPR gRNA library version 1
Pooled Library#67989PurposeKnockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting
Plasmid#156431PurposeTargeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTGDepositorAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#156452PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTGDepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct
Plasmid#156433PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTGDepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LibTest
Pooled Library#113147PurposeCustomized sgRNA library targeting 86 E. coli genesDepositorExpressionBacterialAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_ABE_D3_empty
Plasmid#203809PurposePlasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).DepositorInsertABE_D3
UseCRISPRPromoterCMVAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP
Pooled Library#197349PurposeTo allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.DepositorSpeciesHomo sapiensUseLentiviralAvailable SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP
Pooled Library#197348PurposeTo allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.DepositorSpeciesHomo sapiensUseLentiviralAvailable SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone
Pooled Library#75317PurposeMouse sgRNA library in backbone lentiCRISPRv2 targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controlsDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse Brie kinome pooled library, guides 1-4 in lentiGuide-Puro backbone
Pooled Library#75316PurposeMouse sgRNA library in backbone lentiGuide-Puro targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controlsDepositorAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone
Pooled Library#75314PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone
Pooled Library#75315PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone
Pooled Library#75312PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human Brunello kinome pooled library, guides 5-8 in lentiGuide-Puro backbone
Pooled Library#75313PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Oxford Drosophila melanogaster Genome-wide CRISPR Library
Pooled Library#64750PurposeLibrary designed for genomic targeting and screening in Drosophila cells. All possible gRNA target sites within the shared exonic regions on either DNA strand of the Drosophila genome were computed.DepositorExpressionInsectSpeciesDrosophila melanogaster, Streptococcus pyogenes, and SyntheticUseCRISPRAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Splicing-targeting CRISPR-Cas9 library for human lncRNAs
Pooled Library#119977PurposeDesigned for genomic deletion screening of functional lncRNAs by targeting their splice sites.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only