We narrowed to 3,305 results for: grna design

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Wei lab paired-guide RNA (pgRNA) CRISPR–Cas9 library for human long non-coding RNAs (lncRNAs)

Pooled Library

#89640

Purpose

Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs).

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Expression

Mammalian

Use

Lentiviral

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only
pscAAV-KIKO-U6-gThy1-p2a-mCherry-STOP-pA-400

Plasmid

#249129

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gThy1 cut site, disrupting Thy1 expression (=knockout)

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1, mCherry (Thy1 Mouse, Synthetic)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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dCas9-NS3-NLS/VPR

Plasmid

#112244

Purpose

Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

dCas9-NS3-NLS/VPR

Tags

AU1 (N term on NS3) and HA (C term on NS3)

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PLJR965

Plasmid

#115163

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled Library

#67989

Purpose

Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Knockout library targeting 18,424 mouse genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
pscAAV-KIKO-U6-gTgfbr2-p2a-mCherry-STOPpA-400

Plasmid

#249131

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gTgfbr2 cut site, disrupting Osmr expression (=knockout)

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2, mCherry (Tgfbr2 Mouse, Synthetic)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

Plasmid

#156431

Purpose

Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

RAD21, Halotag (Rad21 Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#156452

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

Depositor

Elphege Nora

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

RAD21, AID (Rad21 Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

Plasmid

#156433

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

SORORIN, AID (Cdca5 Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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LibTest

Pooled Library

#113147

Purpose

Customized sgRNA library targeting 86 E. coli genes

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only
pAcCas12nHS_ABE_D3_empty

Plasmid

#203809

Purpose

Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

ABE_D3

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197349

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75317

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Mouse Brie kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

Pooled Library

#75316

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75314

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone

Pooled Library

#75315

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

Pooled Library

#75312

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only