We narrowed to 31,407 results for: Eng
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Plasmid#223408PurposeT-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT38
Plasmid#223410PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT39
Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT40
Plasmid#223412PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT41
Plasmid#223413PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT42
Plasmid#223414PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT43
Plasmid#223415PurposeT-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.DepositorInsertZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
UseCRISPR; T-dna vectorTagsNLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT06
Plasmid#223378PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS-CMV-mP-luc
Plasmid#213506PurposePositive control vector for luciferase lentivirus-based enhancer assayDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F
Plasmid#200877PurposeZooTsy_TnsF expression construct for protein purification.DepositorInsertZooTsy_TnsF
UseTagsExpressionBacterialMutationTnsF-Y584FPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT384: pSC101-Donor(ZooTsy)
Plasmid#200875PurposeZooTsy donor harboring KanR cargo.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT81: pTarget(ZooTsy)
Plasmid#200876PurposeTarget plasmid containing attachement site for ZooTsy.DepositorInsertZooTsy_comM
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT315: pHelper(ZooTsy)_TnsF-Y584F
Plasmid#200872PurposeInducible expression of ZooTsy proteins.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationTnsF-Y584FPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT375: pDonor(ZooTsy)_excision-reporter
Plasmid#200873PurposeReporter for ZooTsy excision.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT155: pHelper(ZooTsy)_ΔHTH
Plasmid#200868PurposeInducible expression of ZooTsy (except HTH) proteins.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT156: pHelper(ZooTsy)_ΔTnsF
Plasmid#200869PurposeInducible expression of ZooTsy (except TnsF) proteins.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT148: pHelper(ZooTsy)
Plasmid#200866PurposeInducible expression of ZooTsy proteins.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT154: pHelper(ZooTsy)_ΔYRec
Plasmid#200867PurposeInducible expression of ZooTsy (except YRec) proteins.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT368: pSC101-Donor(AjTn6022)
Plasmid#200864PurposeAjTn6022 donor harboring KanR cargo.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF
Plasmid#200865PurposeAjTn6022_TniQ expression construct for protein purification.DepositorInsertAjTn6022_TnsF
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT112: pTarget(AjTn6022)_U50D50
Plasmid#200862PurposeTarget plasmid containing attachement site for AjTn6022.DepositorInsertAjTn6022_comM
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT91: pR6K-Donor(AjTn6022)
Plasmid#200863PurposeAjTn6022 donor harboring KanR cargo.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT385: pHelper(AjTn6022)_QF-separated
Plasmid#200859PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT151: pHelper(AjTn6022)_ΔTnsC
Plasmid#200854PurposeInducible expression of AjTn6022 proteins (except TnsC).DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT152: pHelper(AjTn6022)_ΔTniQ
Plasmid#200855PurposeInducible expression of AjTn6022 proteins (except TniQ).DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT149: pHelper(AjTn6022)_ΔTnsA
Plasmid#200852PurposeInducible expression of AjTn6022 proteins (except TnsA).DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT150: pHelper(AjTn6022)_ΔTnsB
Plasmid#200853PurposeInducible expression of AjTn6022 proteins (except TnsB).DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT147: pHelper(AjTn6022)
Plasmid#200851PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m1
Plasmid#204463PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR A127N mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only