Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 36,099 results for: REP

Clear keywords
Showing: 3301 - 3320 of 36099 results

  1. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep
    #98747-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  5. pUPD2:35S-TMV-Rep-MP

    Plasmid
    #106217
    Purpose
    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)
    Depositor
    Tomáš Moravec
    Insert
    Infectious clone of Tobacco mosaic virus, 5' part
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106217-plasmid-map-sequence-id-208883

    Enlarge

  6. pUPD2:35S-TMV-Rep-MP

    Plasmid
    #106217
    Purpose
    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)
    Depositor
    Tomáš Moravec
    Insert
    Infectious clone of Tobacco mosaic virus, 5' part
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106217-plasmid-map-sequence-id-208883

    Enlarge

  7. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

    Enlarge

  10. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

    Enlarge

  11. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

    Enlarge

  15. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45967-plasmid-map-sequence-id-64561

    Enlarge

  17. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45967-plasmid-map-sequence-id-64561

    Enlarge

  18. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

    Enlarge

Showing: 3301 - 3320 of 36099 results