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pDONR223_XIAP_WT

Plasmid

#82170

Purpose

Gateway Donor vector containing XIAP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XIAP (XIAP Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SAMHD1_WT

Plasmid

#81899

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway Cloning

Mutation

G404E; A546V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MigR1-AE

Plasmid

#12431

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223_TNFRSF17_WT

Plasmid

#82263

Purpose

Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF17 (TNFRSF17 Human)

Use

Gateway Cloning

Mutation

N81S; K86E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3F

Plasmid

#224447

Purpose

Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHASW insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV3A

Plasmid

#224442

Purpose

Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYVGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 a-syn A53T D115A mNeonGreen-3K-B11

Plasmid

#232017

Purpose

Bacterial expression of alpha synuclein A53T mutant, tagged with the final beta strand of mNeonGreen-3K

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

SNCA (SNCA Human)

Tags

mNeonGreen-3K beta11

Expression

Bacterial

Mutation

Ala53Thr Asp115Ala

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-3XHA

Plasmid

#232003

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B-d55-96

Plasmid

#232013

Purpose

Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_SDHA_WT

Plasmid

#82278

Purpose

Gateway Donor vector containing SDHA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SDHA (SDHA Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SOCS3_WT

Plasmid

#82245

Purpose

Gateway Donor vector containing SOCS3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SOCS3 (SOCS3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_MLST8_WT

Plasmid

#82258

Purpose

Gateway Donor vector containing MLST8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MLST8 (MLST8 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE-4A6-vioC

Plasmid

#73439

Purpose

Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 and P4A6 (orthogonal T7-lac variants)

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCA_WT

Plasmid

#82225

Purpose

Gateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCA (PRKCA Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_TBK1_WT

Plasmid

#82285

Purpose

Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBK1 (TBK1 Human)

Use

Gateway Cloning

Mutation

N388D; K570Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV1E

Plasmid

#224439

Purpose

Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDTMSK insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM10_WT

Plasmid

#81958

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_IRGM_WT

Plasmid

#82134

Purpose

Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRGM (IRGM Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-FLAG-human cGAS-HA

Plasmid

#186849

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

FLAG and HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-GFP-dynein-2(D1091–Q4307)

Plasmid

#64064

Purpose

Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

Depositor

Andrew Carter

Article

Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

Insert

Cytoplasmic dynein-2 (DYNC2H1 Human)

Tags

GFP, His tag, and ZZ tag

Expression

Insect

Mutation

Deleted amino acids 1-1090, extra C-terminal vali…

Promoter

Polyhedrin

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PIK3CD_WT

Plasmid

#82222

Purpose

Gateway Donor vector containing PIK3CD , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CD (PIK3CD Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPOP_p.F133S

Plasmid

#81637

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway Cloning

Mutation

F133S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PPP2R1A_WT

Plasmid

#81760

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RIMS2_WT

Plasmid

#81803

Purpose

Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RIMS2 (RIMS2 Human)

Use

Gateway Cloning

Mutation

S86F; Q295R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPOP_p.W131G

Plasmid

#81632

Purpose

Gateway Donor vector containing SPOP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway Cloning

Mutation

W131G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_CYP3A5_WT

Plasmid

#81823

Purpose

Gateway Donor vector containing CYP3A5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYP3A5 (CYP3A5 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-MyoAAV1C

Plasmid

#224438

Purpose

Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLSTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PHR-GFP-FUSN-VP16

Plasmid

#183933

Purpose

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KIR3DL2_WT

Plasmid

#81773

Purpose

Gateway Donor vector containing KIR3DL2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KIR3DL2 (KIR3DL2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPOP_p.F102C

Plasmid

#81642

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway Cloning

Mutation

F102C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-mCherry-DMDEx23-eGFP

Plasmid

#211366

Purpose

Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Article

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23-eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-APEX2

Plasmid

#215127

Purpose

Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX2 (APEX2 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

Plasmid

#185915

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_UNC93B1_WT

Plasmid

#81977

Purpose

Gateway Donor vector containing UNC93B1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

UNC93B1 (UNC93B1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SERPINB4_WT

Plasmid

#81766

Purpose

Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SERPINB4 (SERPINB4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPOP_p.E50K

Plasmid

#81631

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway Cloning

Mutation

E50K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBactin-EB3-mNeonGreen

Plasmid

#196848

Purpose

labelling of microtubule plus ends

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EB3-NeonGreen (MAPRE3 Human)

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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