We narrowed to 4,371 results for: Lta;

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zmiRFP

Plasmid

#108415

Purpose

Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zmiRFP

Use

Zebrafish

Promoter

UAS

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP (Kcnj2 Mouse)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-miRFP

Plasmid

#108416

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

Plasmid

#159086

Purpose

Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

Depositor

Jeremy Day

Article

Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

Insert

empty crRNA-acRNA backbone, dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

hSYN, U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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cacnb4 (rat) in pMT2 vector

Plasmid

#107426

Purpose

Expresses Calcium channel beta4 auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

Insert

cacnb4 (Cacnb4 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

Plasmid

#102596

Purpose

Lentivector to express STING V155M delta 368 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 368

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-N1

Plasmid

#108409

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA D122A pcDNA3

Plasmid

#206120

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA E1658A (EIVA) pcDNA3

Plasmid

#206114

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

E1658A

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 EIVA (E1707A) pcDNA3

Plasmid

#206125

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Mutation

E1707A

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA EIA (E320A) pcDNA3

Plasmid

#206124

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Mutation

E320A

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Article

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only