We narrowed to 7,308 results for: RAP

1. MTK1_028

   Plasmid

   #123690

   Purpose

   Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L6-F1 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK1_029

   Plasmid

   #123691

   Purpose

   Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L7-B2 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPI180

   Plasmid

   #113222

   Purpose

   generic knockin vector for C-terminal tagging with GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Generic knockin vector for c-terminal tagging wit…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. V32M,L84M CoV-2 ORF8

   Plasmid

   #159934

   Purpose

   Protein overexpression in E.coli

   Depositor

   James Hurley

   Article

   Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   CoV-2 ORF8 18-121 (ORF8 CoV-2)
   

   Use

   Tags

   6x Histidine, TEV cleavable

   Expression

   Bacterial

   Mutation

   V32M, L84M

   Promoter

   T7

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-HDR-sphys-3XHA

   Plasmid

   #163690

   Purpose

   plasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene

   Depositor

   Bernardo Sabatini

   Article

   Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

   Insert

   triple HA tag insertion into endogenous mouse synaptophysin gene
   

   Use

   AAV

   Tags

   triple HA tag

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOVC1_AtPHYB-S_X

   Plasmid

   #131183

   Purpose

   Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   AtPHYB-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOVC1_NcVVD-LOV_X

   Plasmid

   #131176

   Purpose

   Expresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   NcVVD-LOV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL729

   Plasmid

   #140399

   Purpose

   Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-cadO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEG R3-L4 #BGV194

   Plasmid

   #48954

   Purpose

   For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRoCascade_gfp

    Plasmid

    #178771

    Purpose

    Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRoDonor

    Plasmid

    #178773

    Purpose

    Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

    Depositor

    Chase Beisel

    Article

    Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

    Insert

    RoCAST left end, cam, RoCAST right end from Rippkaea orientalis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK6_016

    Plasmid

    #124058

    Purpose

    Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    WPRE (extended)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJBL718

    Plasmid

    #140388

    Purpose

    Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-mobO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK2_024

    Plasmid

    #123716

    Purpose

    Encodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pPGK1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Crippled Kozac sequence

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pES002 (pTet-qacR wt)

    Plasmid

    #69031

    Purpose

    wild-type qacR downstream of a Tet promoter

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Insert

    qacR (qacR Synthetic)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. cp-DdCBE G1333-C

    Plasmid

    #174557

    Purpose

    Chloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

    Depositor

    Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

    Insert

    DdCBE G1333-C
    

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    PcUbi

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330 EQR

    Plasmid

    #101733

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRUSH

    Plasmid

    #54479

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

20. p3E-Dam-myc no-pA

    Plasmid

    #82600

    Purpose

    3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Dam-Myc (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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