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We narrowed to 14,357 results for: cas9

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Showing: 3321 - 3340 of 14357 results

  3. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  6. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  7. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  8. ciCas9(F22)_pcDNA5

    Plasmid
    #100552
    Purpose
    Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
    Depositor
    Dustin Maly
    Publication
    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ciCas9(F22)
    Use
    CRISPR; Frt, flp-in
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100552-plasmid-map-sequence-id-193861

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  9. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47869
    Purpose
    All-in-one plasmid targeting tdTomato
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47869-plasmid-map-sequence-id-68867

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  10. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid
    #136656
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136656-plasmid-map-sequence-id-275491

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  11. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  12. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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  14. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid
    #228718
    Purpose
    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nme2SmuCas9-E932R nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228718-plasmid-map-sequence-id-453994

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  16. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  18. PX458-3xHA-SpCas9

    Plasmid
    #130968
    Purpose
    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xHA-NLS-SpCas9
    Use
    CRISPR
    Tags
    3x HA, EGFP, and NLS
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Nov. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130968-plasmid-map-sequence-id-262662

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  20. AID-C12*-dCas9-BE4max

    Plasmid
    #216731
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-dCas9-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216731-plasmid-map-sequence-id-429722

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Showing: 3321 - 3340 of 14357 results