We narrowed to 15,991 results for: LEA

1. pB5_R8-3i-ATP8_v8_AGKS

   Plasmid

   #191510

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE R8-3i-ATP8 AGKS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-FB-orig BA

   Plasmid

   #13420

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   orig BA
   

   Tags

   Gal11P

   Expression

   Bacterial

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. PML-3/pSG5

   Plasmid

   #50939

   Purpose

   expression of PML with Sumo sites destroyed

   Depositor

   Pier Pandolfi

   Article

   Zhong et al Blood. 2000 May 1;95(9):2748-52.

   Insert

   PML-3 (PML Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K65R, K160R, K490R (Sumo modification sites)

   Promoter

   SV40

   Available Since

   Jan. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCS2 Notch1 ICv-6MT

   Plasmid

   #41730

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Intracellular Domain (Notch1 Mouse)
   

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 protein beginning at Vali…

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A-1x4

   Plasmid

   #58768

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-HIRA-polyA

   Plasmid

   #59781

   Purpose

   Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT3928

   Plasmid

   #113331

   Purpose

   control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Use

   Unspecified

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUNIV-EGFP

    Plasmid

    #24706

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Insert

    EGFP (eGFP Aequoria victoria)
    

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    Dec. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

    Plasmid

    #205278

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Tags

    TagRFP

    Expression

    Plant

    Promoter

    CaMV 35 promoter

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1-14-3-3 gamma GST

    Plasmid

    #13280

    Depositor

    Michael Yaffe

    Article

    Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pJH726_pJH_LldR_PlldR-sfGFP.15TAG

    Plasmid

    #163730

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.15TAG
    

    Expression

    Bacterial

    Mutation

    gfp with first 15 leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH773_pTech_PlldR-HHRz-supP(opal)

    Plasmid

    #163732

    Purpose

    Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    hhrz-supP
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. KBB460

    Plasmid

    #185094

    Purpose

    NMA111-GFP wild type under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111-GFP

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG.CenpB(FL)-GFP-Halo

    Plasmid

    #176205

    Purpose

    Centromeric anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    CenpB-GFP-Halo
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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