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We narrowed to 6,059 results for: actin

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Showing: 321 - 340 of 6059 results

  1. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid
    #73498
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73498-plasmid-map-sequence-id-364403

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  7. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (AAV5)

    Viral prep
    #84445-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (#84445). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] plasmid DNA. CAG-driven, Cre-dependent Jaws-GFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    CAG
    Tags
    GFP (Cre-dependent)
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Publication
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40132-plasmid-map-sequence-id-51020

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  10. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Publication
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40131-plasmid-map-sequence-id-51019

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  11. pCIS GEM-CEPIA1er

    Plasmid
    #58217
    Purpose
    Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum
    Depositor
    Masamitsu Iino
    Publication
    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEM-CEPIA1er
    Tags
    myc
    Expression
    Mammalian
    Mutation
    GEM-GECO1 E298D F359W D400E E407D
    Promoter
    CAG
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58217-plasmid-map-sequence-id-89557

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  12. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Publication
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141347-plasmid-map-sequence-id-296391

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  13. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Purpose
    Cre-dependent AAV-mediated expression of tdTomato under the CAG promoter
    Depositor
    Edward Boyden
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.S, AAV PHP.eB, and AAV Retrograde
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    28306-plasmid-map-sequence-id-520301

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  14. AAV pCAG-FLEX-EGFP-WPRE (AAV Retrograde)

    Viral prep
    #51502-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Publication
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    CAG
    Tags
    EGFP (Cre-dependent)
    Available since
    June 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

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  18. pAAV-CAG-GFP (AAV1 trial size)

    Viral prep
    #37825-AAV1.T
    Purpose
    Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-CAG-GFP (AAV5)

    Viral prep
    #37825-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 321 - 340 of 6059 results