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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,384 results for: actin

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Showing: 321 - 340 of 3384 results

  1. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid
    #191334
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191334-plasmid-map-sequence-id-381755

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  2. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid
    #104879
    Purpose
    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
    Depositor
    Christopher Potter
    Insert
    CD-dCas9(m4)-UDI
    Use
    CRISPR
    Expression
    Insect
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104879-plasmid-map-sequence-id-209447

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  3. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

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  5. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid
    #196854
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196854-plasmid-map-sequence-id-391811

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  9. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid
    #196853
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196853-plasmid-map-sequence-id-390738

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  16. pBetaActin-KIF13aMotor-mScarlet-FKBP

    Plasmid
    #191337
    Purpose
    Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    KIF13aMotor-mScarlet-FKBP (KIF13A Human)
    Expression
    Mammalian
    Mutation
    motor domain aa 1-639
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191337-plasmid-map-sequence-id-381769

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  19. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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Showing: 321 - 340 of 3384 results