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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,850 results for: nin

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Showing: 321 - 340 of 66850 results

  3. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52970-plasmid-map-sequence-id-82408

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  12. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106280-plasmid-map-sequence-id-512176

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  18. pcDNA3.1(+)/Luc2=tdT

    Plasmid
    #32904
    Purpose
    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
    Depositor
    Christopher Contag
    Publication
    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    Tags
    tdTomato red fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV-IE and n/a
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32904-plasmid-map-sequence-id-229770

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Showing: 321 - 340 of 66850 results