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We narrowed to 10,856 results for: kars;

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pBabe JunD-Venus puro

Plasmid

#58496

Purpose

mammalian expression of Venus-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Use

Tags

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-mM₃O-IP

Plasmid

#46644

Depositor

Nobuaki Kikyo

Article

Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

Insert

mM₃O (Pou5f1 Mouse)

Use

Retroviral

Tags

IRES Puro and mouse MyoD (aa 1-62)

Expression

Mammalian

Mutation

contains a fragment of mouse MyoD (aa1-62) fused …

Promoter

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX FynT-SH2

Plasmid

#46430

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynB/FynT (FYN Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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CD8-dCherry-FRB

Plasmid

#100739

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Use

Tags

FRB and dCherry

Expression

Mammalian

Mutation

Dark mCherry (K70N) non-fluorescent

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDR1__PAK1

Plasmid

#116813

Purpose

Lentiviral expression of DDR1__PAK1 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDR1__PAK1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-FLAG-YWAA-pGK-HYG

Plasmid

#107375

Purpose

Lentiviral expression of FLAG-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAR69_STRI_m(KS_MAT)_H8_pET22b

Plasmid

#122849

Purpose

Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

m(KS_MAT) (Fasn Mouse)

Use

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-LITAF

Plasmid

#178444

Purpose

doxycycline-inducible expression of Human LITAF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

LITAF (LITAF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG R106L-CPTP

Plasmid

#170744

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R106L

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG K60A-CPTP

Plasmid

#170743

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K60A

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Abl-SH2 RK

Plasmid

#46403

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl RK (Abl1 Mouse)

Use

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 139-239

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Jak1-SH2

Plasmid

#46447

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak1 (JAK1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 430-552

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg2(NC)-SH2

Plasmid

#46472

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg2(NC) (PLCG2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 524-749

Promoter

Tac

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-ATP2B4__OPTC

Plasmid

#116808

Purpose

Lentiviral expression of ATP2B4__OPTC fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ATP2B4__OPTC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Grb10-SH2

Plasmid

#46437

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb10 (GRB10 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 435-548

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERGIC1__RANBP17

Plasmid

#116816

Purpose

Lentiviral expression of ERGIC1__RANBP17 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERGIC1__RANBP17

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(C)-SH2

Plasmid

#46431

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(C) (RASA1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 339-452

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA 27

Plasmid

#132396

Purpose

Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-MCM5__LYN

Plasmid

#116828

Purpose

Lentiviral expression of MCM5__LYN fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MCM5__LYN

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp2-SH2

Plasmid

#46460

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp2 (BCAR3 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 144-259

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA1_P2A_Hygro_Barcode

Plasmid

#120451

Purpose

Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-WNK1__PRMT8

Plasmid

#116845

Purpose

Lentiviral expression of WNK1__PRMT8 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WNK1__PRMT8

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin2-SH2

Plasmid

#46416

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin2 (CHN2 Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 49-155

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CDC42BPB__RGS6

Plasmid

#116810

Purpose

Lentiviral expression of CDC42BPB__RGS6 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDC42BPB__RGS6

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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