We narrowed to 22,823 results for: Sis

1. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB59 pDEST crybb1:mKate2

   Plasmid

   #195986

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Markus Affolter

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Use

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available Since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pDGE337

   Plasmid

   #153246

   Purpose

   M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGE497

   Plasmid

   #153249

   Purpose

   M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSH824-ssr

   Plasmid

   #198027

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Apr resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AprR promoter

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE342

   Plasmid

   #153251

   Purpose

   M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPML1 (GB0045)

   Plasmid

   #68164

   Purpose

   Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtML1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNat-AID*-9myc

   Plasmid

   #99523

   Purpose

   C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDB179-TfCut2-S121P-D174S-D204P

    Plasmid

    #218695

    Purpose

    Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    S121P-D174S-D204P

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSE901

    Plasmid

    #91709

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJY-RpABE

    Plasmid

    #112872

    Purpose

    binary vector for ABE expression in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. peCALWY-4

    Plasmid

    #22236

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C418S mutation linker consists of 9 GGSGGS repea…

    Promoter

    Available Since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMCtKq28-TSC10M1-sspB

    Plasmid

    #49526

    Purpose

    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMCtN-TSC10M1-sspB

    Plasmid

    #49521

    Purpose

    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIB81-pmGFP

    Plasmid

    #75366

    Purpose

    Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    prenylated EGFP

    Expression

    Mammalian

    Mutation

    residues 1-81 of CIB1

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFGC5941-PacI-ath-STTM165/166-48nt

    Plasmid

    #84091

    Purpose

    target miRNA165/166 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM165/166
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA NIA Null

    Plasmid

    #198728

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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