166,818 results

1. miniCMV-tdTomato-tDeg

   Plasmid

   #217521

   Purpose

   Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   tdTomato-tDeg fluorogenic protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JM83

   Bacterial Strain

   #50348

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Messing et al Recombinant DNA Technical Bulletin, 1979

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available Since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-sfCherry11x4-ß-actin

   Plasmid

   #70220

   Purpose

   Expresses sfCherry11x4-ß-actin in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry11x4-actin (ACTB Human)
   

   Use

   Tags

   sfCherry11x4

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-OptoTGFBRs

   Plasmid

   #118942

   Purpose

   Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage-cmv-cfp-24xpp7

   Plasmid

   #40652

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   CFP-24xPP7
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

   Plasmid

   #229678

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold, HA

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1-mitoRexYFP

   Plasmid

   #60246

   Purpose

   Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

   Insert

   mitoRexYFP
   

   Use

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

   Plasmid

   #176271

   Purpose

   Expression of CasMINI-V3.1 in mammalian cells.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI-V3.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-mTurquoise2-IL-1beta-mNeonGreen

   Plasmid

   #166783

   Purpose

   FRET analysis (pro-IL-1beta cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-IL-1b-mNeongreen (IL1B Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_FGFR3_TACC3

    Plasmid

    #205821

    Purpose

    Express mEGFP-tagged fusion protein, FGFR3_TACC3 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    FGFR3_TACC3 (FGFR3, TACC3 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 768 pME mApple-CAAX

    Plasmid

    #195961

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple-CAAX (membrane)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

    Plasmid

    #101852

    Purpose

    Direct reprogramming of adult fibroblasts to neurons

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    Ascl1, Brn2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6, PGK

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiGuide_DKO

    Plasmid

    #183193

    Purpose

    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-hAIM2

    Plasmid

    #51542

    Purpose

    Express GFP-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. STICR_Index3

    Pooled Library

    #186335

    Purpose

    High Complexity Lentiviral Barcode Library

    Depositor

    Tomasz Nowakowski

    Article

    Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pBbS8a-RFP

    Plasmid

    #35274

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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