We narrowed to 15,248 results for: Tec;

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pIDS-LSD1fl

Plasmid

#109157

Purpose

Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Tags

SNAPf-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Keratin-17

Plasmid

#54134

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Keratin (KRT18 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Tog

Plasmid

#29480

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K19R, E118G, G254R, K1834R

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-LIC1

Plasmid

#66600

Purpose

Expresses Dynein-1 LIC1 as a mGFP fusion

Depositor

David Stephens

Insert

LIC1 (DYNC1LI1 Human)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

Plasmid

#133794

Purpose

U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

NmeCas9 and Nme2Cas9 sgRNA entry vector

Expression

Mammalian

Mutation

sgRNA architecture from Amrani et al. Genome Biol…

Promoter

U6

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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phNFL

Plasmid

#132589

Purpose

Expresses human NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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IKK2-EGFP

Plasmid

#111195

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

Insert

IKKbeta (IKBKB Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-GS1

Plasmid

#89454

Purpose

Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(C358S)

Plasmid

#42513

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Cys358->Ser

Use

Retroviral

Tags

Flag

Mutation

Changed Cysteine 358 to Serine

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pJT119b

Plasmid

#50551

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pPAS-E

Plasmid

#39867

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein PAS domain

Tags

E coil

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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